BUREAU OF ANIMAL INDUSTRY. 489 



other (No. 2) died on tlie fifth day, the third (Xo. 4) on the seventh day 

 after iuoeulation. 



In the hiugs and spk'iMi of ISTo. 1 a moderate number of bacilli were 

 fouud, while cover-glass preparations of kiducy and liver showed none. 

 The bacilli vaiied in length from 2 to 10 micromillimeters, and were 

 about .G micromillimeter broad; ends rounded. Mouse Ko. 2 died of 

 malignant oedema. The kidney of the third one (No. 4) contained large 

 numbers of the oval bacteria, found in cover-glass preparations of tbo 

 spleen in pigs affected with swine plague later on, and identitied as the 

 cause of swine plague. The paler center was very distinct, suggesting 

 very strongly the appearance of spores. This was the first time these 

 bacteria were seen, since the cover-glass preparations made irom the 

 organs of pig No. 105, whence these mice had been inoculated, proved 

 entirely negative. The cultures, both solid and liquid, obtained from 

 the heart's blood of these mice proved, as might Isave been expected, 

 to be mixtures of several kinds of bacteria. The gelatine invariably 

 became liquid. For want of time they were set aside. Two mice, in- 

 oculated with a piece of kidney of mouse No. 4, November 12, were 

 quite sick November 14 and killed with chloroform, but no bacteria 

 tound in the organs of either animal. The two mice inoculated with a 

 bit of spleen seemed well about a week after. They were then removed 

 from the jar in which they had been placed after inoculation and placed 

 in a larger cage with other healthy mice. A few days after one was 

 fouud dead, bat owing to want of time was not examined. About fif- 

 teen days after the date of inoculation the other was found dead. On 

 examination the site of inoculation was healed over. The spleen was 

 found enlarged to four or five times its normal size, its color resembling 

 that of striped muscle. The surface of the kiducy contained a few 

 whitish patches. Liver with a tape- worm cyst. Lungs slightly con- 

 gested. In the kidney numerous oval bacteria were fouud, precisely 

 similar to those found in mouse No. 4 and in the spleen of the deceased 

 pigs. In the spleen the bacteria were of the same size, but no pale 

 center was noticed. Our attention was now aroused to the long i)eriod 

 of this disease in mice accompanied by this bacterium, which was q^ar- 

 acterized by its elongated, oval form and its pale center when stained 

 a few minutes with an aqueous solution of methyl-violet. 



The other pig (No. lOG), brought to the station with No. 105, died in 

 the night between the 0th and 7th of November. T^e weather was 

 warm ami favorable to decomposition. On examining the viscera in 

 the morning the lesions were found somewhat dillerent from those of 

 pig No. 105. The lungs were apparently intact. In the digestive tract 

 a i)atch of the mucous membrane of the stomach was deeply inllamcd, 

 presenting an intensely red color. The ileum, though severely intlamed, 

 was free from ulcerations. In the large intestine there were three or 

 four ulcerated patches about an inch square near the valve. The rau- 

 cous membrane in the remainder of the intestine was studded with 

 closely-set, blackish, depressed spots, evidently old extravasations, and 

 about to become ulcers. In this animal the lesions were not so far ad- 

 vanced as in the previous case- 

 Cultures in gelatine and liquid media of the spleen and blood from 

 the heart remained sterile, with the exception of one colony in the gela- 

 tine culture of the spleen. Cover-glass preparations were likewise 

 negative. 



We will now return to those animals which had contracted swine 

 plague in spite of vaccination. The ^jos^ moriem apiiearances of these 

 animals (95, 10.3, 94) have already been given on page 485. In cover- 



