BUREAU OF- ANIMAL INDUSTRY. 491 



the direct effect of tlio virus aud were sufferiiijj from the indirect re- 

 sults tbereof. Tbe i>rese:ice of lari^o tumors at the poiutj* of inocula- 

 tion indicates s^'cnter |)Ower of resistance on the part of these two ani- 

 mals than was shown by animals subsequently inoculated. 



The healthy check-pig: (No. 110) penned with those died December G 

 after four or five days of illness, durinq; which period the feces were at 

 times covered with blood. The body was examined December 7. Skin 

 not discolored; the inguinal glands much congested ; surface of spleen 

 covered Avith nnmerous red punctiform elevations; right heart dis- 

 tended with a dark semisolid clot; two or three punctiforni extravasa- 

 tions on the auricular appendages; lung- worms quite numerous; lungs 

 apparently normal, however; stomach deeply con^-ested in fundus; 

 glands of mesentery much swollen, those ot rolon dark red; the mu- 

 cosa of large intestine intensely intlamed throu;j[liout; no ulceration; 

 surface of both kidneys dotted with numerous daik roil ])oints; medulla 

 very dark. Cover-glass preparations of the sjileen contain the oval bac- 

 terium in small numbers. A gelatine tube culture of spleen and one of 

 blood from the heart contains the same motile bacterium alone. 



From tlie foregoing description avo observe that (lie check-animal died 

 from a very acute attack, and it seems leasoiiabU; to su]>pose that it 

 caught the disease from the two inoculated aniiiiais, and, being the more 

 susceptible, quickly succumbed to the virus. The herd Inun which this 

 animal was taken did not allow any suspicion as ro its soundness con- 

 sidering later observations. It is highly |)robable that the disease ap- 

 peared early in the inoculated amimals, but owing to its comparative 

 mildness remained unnoticeil for a time. The cultures from Xo. 110 

 were successful in demonstrating the ])reseuce of the oval motile bac- 

 terium. Compare with this the negative results of the chronic cases. 



Two mice, inoculated November 10 as above, with a l>it of si)leen from 

 pig No. 04, were found dead L)e(;ember G. .uu' of them drowned. The 

 other had a very large spleen while the kil-i^-v was quite i)ale. A cov- 

 er-glass preparation each of spleen, kiihu-.v, :iii;l liver, contained no bac- 

 teria. A liquid culture from blood eunfained the oval bacterium, pure, 

 however, November iM two mice were inoculated hypodermically with 

 about live drops bf a liquid cnltuie of the bioud of jiig No. 01. Novem- 

 biT 28 one was found dead. The lungs were very much congeste<l and 

 sank iu water. No l»acteiia disceriuble in cover glass preparations of 

 spleen, kidjieys. and lungs. The second oru' died December 3; spleen 

 enlarged, medulla of kidney very daik, lungs cedematous, reddish. The 

 characteristic oval bacterium x)resent in very large" numbers in cover- 

 glass preparations of spleen, kidney, liver, lungs, and heart's blood. A 

 liquid culture from the same found ])ureAvheu tested on gelatine ]>lates. 



Two pigs (Nos. 00, 07) were fed Novend)er 18 with the intestines of 

 No. 0-1. No. 00 had a tem])erature of l(».")-p F. on November 21; <liar- 

 rhea set in on the following day and contiiiued until death, which oc- 

 curred quite unexpectedly on November 21. The pout mortem appear- 

 ances were in brief as follows: 



Superficial inguinal glands very large, (edematous. In the abdomen 

 a considerable (piantity of straw-colored tiuitl. Vessels of stomach, mes- 

 entery, aiul large inieytine very full; glands of mesentery consideraVdy 

 enlarged, somewhat reddened. A fev,- jjunctiform extravasations on 

 right auricle. Scattered extravasations underpulmonary pleura. Bron- 

 chial and mediastinal glands enlarged and very dark red. Mucous 

 membrane of caecum of a dull purplish hue with very thin scattered 

 patches of necrosed tissue. At the base of the valve the tlask-shai)ed 

 glands distended with yellowish-white plugs. 



