492 REPORT OF THE COMMISSIONER OF AGRICULTURE. 



is"o. 97 bad diarrliea on I^ovember 25 ; the feces mixed with blood on 

 Isovember 27 ; fouud dead ou the following- day. In this animal there 

 were, in addition to the lesions above described, a diffused reddening of 

 the subcutaneous fatty tissue with numerous puuctiform extravasations. 

 Tiie surface of the spleen was dotted with slightly-raised, blood-red 

 points most numerous near the border 5 liver much enlarged, borders 

 very blunt. On section some acini were much more congested than 

 neighboring ones; medulla of kidneys, even the papillae, deeply red- 

 dened. Beside the dusky hue of the mucosa of the large intestine, the 

 surface near the rectum was covered with firmly adherent coagnla, which, 

 on being removed, e:^ posed a dark red surface dotted with darker points. 

 The contents of large intestine blackish, as if mingled with blood. 

 A deeply congested patch of mucous membrane along greater curva- 

 ture of stomach which contained several blood clots. Cover- glass prep- 

 arations of spleen and liver of both animals contained the same char- 

 acteristic bacterium found in Nos. 94, 95, 103 ; most abundant in the 

 s])leen ; tliosc of blood were negative as usual. A culture made in a 

 tube of gelatine by dipping a platinum wire into the peritoneal exudate 

 of No. 90 and then piercing the gelatine with it, remained sterile, al- 

 though it was pierced three times. A culture inoculated in the same 

 way with blood from the heart contained a moderate number of minute, 

 uniform colonies in each track, while another, inoculated by piercing 

 the spleen, contained a large number of colonies in each track. Cult- 

 ures in liquid media, two inoculated with blood from the heart, one 

 from the spleen, one from the peritoneal iluid, and one from an inguinal 

 gland, exhibited the usual features of pure cultures of the bacterium 

 on the following day. This was confirmed by finding them ail motile, 

 and when line cultures were made on gelatine plates of each, all the 

 colonies derived therefrom resembled oue another, as well as those of 

 the previous cases. All cultures inepared from this case therefore were 

 pure and alike. 



Cultures in tubes of gelatine of heart's blood and spleen of ^0. 97 

 were equally successful, that of blood containing a moderate number,^ 

 that of spleen a very large number of colonies. Two liquid cultures of 

 the blood, one from the spleen and one from an inguinal gland, con- 

 tained the same motile bacteiium in a pure condition as shown by line 

 cultures. 



In these, as in many preceding and subsequent experiments, we take 

 pleasure in pointing out the fact that licpiid cultures are not to bo con- 

 demned, for with pro])er tubes and snilieient care pure cultures are in- 

 variably obtained, and as sueli the contained bacteria are far more ac- 

 cessible to study than those buried in the dei)ths of the gelatine. 



A bit of spleen from pig No. 90 was put under the skin of two mice 

 November 25. One of them was iound dead December 7. Preoisely 

 the same appearances as in the mouse inoculated with a culture from 

 pig No. 94. The si)leen was very large. The oval bacterium was found 

 abundantly in the si)]een, kidney, and liver. The other mouse died on 

 I)eee;nber 12. The lesions were the same and the bacteria were abun- 

 dant in the spleen. No cultures prepared. 



On December 3, three mice (Nos. 31, 32, and 33) were inoculated hypo- 

 dermicallv with 5 to 10 drops of a liquid culture from the spleen of pig 

 No. 97. No. 31 fouud dead December 8. No marked lesions, excepting 

 congestion of lymphatic glands of knee-fold. A liquid culture from the 

 heart, when tested by line cultures, was found to consist of the bacteri- 

 um injected and another form rapidly liquefying gelatine, 



Nos. 32 and 33 died the same day. In the former the spleen was but 



