BUEEAU OF ANIMAL INDUSTllY. 495 



moderately enlarged ; lungs somewhat congested. Bacterium in cov- 

 ers of spleen, kiduej', liver, and lungs. A licjuid culture of blood from 

 the heart was ibund pure. In No. 33 the s]jleeu was very large, medulla 

 of kidney very dark red. Several highly-congested areas of lungs ; 

 bacterium abundant in spleen ; few in kidney, liver, and lungs. A liquid 

 culture of blood from the heart found pure as above. 



Two mice (Xos. 29 and 30) received under the skin of the back a bit 

 of spleen from pig No. 97 on November 28. Both were alive and well 

 December 22, when they were killed with chloroform. The spleen of one 

 was but slightly enlarged ; that of the other was from three to four 

 times its normal size. No bacteria were found in either. 



There Avas but one rabbit at our disposal at the time of these experi- 

 ments. This animal had been at the station tor many months. On No- 

 vember 27 it was iuoculnted by a hyj^odermic injection into each thigh 

 of about 1*=*^ of a liquid culture i'rora the sjileen of pig No. 9G, found pur(\ 

 It was found dead December I. On examination the spleen was found 

 enlarged, very dark, and friable'; substance of liver and kidneys equally 

 so. lvif;ht heart filled with a dark clot. Left heart contained a small 

 white clot extending into the large vessels. The stomach contained two 

 dark clots, each as large as a walnut. In the middle region, along the 

 greater curvature, the mucous membrane was intensely reddened and 

 dotted with dark spots of extravasated blood, the probable place of the 

 hemorrhage. Cover-glass preparations of the spleen contain immense 

 numbers of the oval bacterium, the liver less, the kidney still less. 

 They were also found in the lungs. Gelatine-tube cultures of the spleen 

 and blood from the heart grew precisely' as the former cultures. Two 

 liquid cultures each of spleen and blood contained the motile bacte- 

 rium. Line cultures on gelatine proved each of them pure. This case 

 seemed so conclusive that the want of additional rabbits was not felt 

 so seriously at the time, although it would have been of great Interest 

 to determine whether the hemorrhage into the stomach is a constant phe- 

 nomenon, since it is not infrequent in pigs, while inflammatory affec- 

 tions of the mucosa seem to be the rule in acute cases. 



Two pigeons were inoculated December 3 with a liquid culture from 

 the spleen of pig No. 97. No. 1 received about ^''° under the skin of 

 the right shoulder. No. 2 received about ^'^^ under the same place and 

 in addition l*^*' beneath the skin near the keel of the sternum. No. 2 

 died within twenty-four hours. There were no lesions perceptible, ex- 

 cepting a deeper red of the pectoral muscle at the point of inoculation. 

 Cover-glass preparations of spleen, liver, and kidney negative. As 

 might have been expected from such a large dose, the bacterium was 

 present in the viscera and blood, as determined by i)ure liquid cultures 

 of blood from heart and liver tissue. No. 1 appeared ill on the follow- 

 ing day. It remained quiet on its perch, with feathers ruftled; feces 

 entirely white until December 8, when blood was passed, mixed with 

 mucus. This condition lasted until December 12, when it was found 

 dead. There was no somnolence noticed at any time, and symptoms 

 pointing to an implication of brain and spinal cord absent. 



On examination, the right pectoral muscle appeared as if the blood 

 had been soaked out and the muscle boiled. Its consistence was that 

 of boiled flesh. This condition prevailed over the major part of .the 

 pectoral muscle for a depth of about three-quarters of an inch. Liver 

 dark and very soft,: spleen and lungs nornuil in appearance. The large 

 intestine for a distance of about G inches from cloaca appeared distended 

 and covered with yellowish spots about one-eighth of an inch in diam- 

 eter. The greater part of the mucous surface was covered with a dark 



