494 KEPOET OF THE COMMISSIONER OF AGRICULTURE. 



Lrowuisli necrosed layer interspersed with yellowish masses. Cover- 

 glass ]irepai-ations of the spleen, liver, and lungs contained large num- 

 bers of the oval bacreriuJii ; very few in blood of heart and in kidney. 

 Two cultures in tubes of luitritive gelatine, one inoculated with blood 

 from the heart, the other from the liver, showed the characterisi ic growth 

 of the bacteriuui after two days. The colonies were very numerous and 

 crowded in the culture from the liver; few and scattered in that from 

 the blood. Tsvo tubes of meat infusiou peptone inoculated, one from 

 the lister the other from the blood, were found pure cultures of this 

 motile bacterium when grown on gelatine plates. 



On iS^ovember 27 two pigs (So». Ill, 114) Avere inoculated by a sub- 

 cutaneous injection of a liquid culture made from the spleen of pig No. 

 90, and found to consist of only one kind of microbe, when tested by line 

 cultures on gelatine plates. The microbe was motile, grew on gelatine 

 like that isolated from previous cases, and was i)resuniably the microbe 

 of swine plague. The culture medium consisted of a sterilized infusion 

 of beef with 1 per cent, peptone. About 1.5<=*^ were injected into each 

 thigh. On December 5 the temi)erature of one of the animals (No. 114) 

 was 103|° F. Bowels somewhat loose at llrst. Ap]>etite good until 

 death, which took i)lace between 9 and 12 a. m., Deeeuiber G, about nine 

 days after inoculation. 



The examination was made December 7, a tera]ierature below ivcez- 

 ing preventing jW6';( ?»oj'/c>?i changes. The su])erticial inj^uinal glands 

 were found considerably swollen, the section dotted with led points 

 and linos. The spleen was somewhat enlarged and darker than nor- 

 mally. The right heart distended with dark, imperfectly coagulated 

 blood. On both auricular appendaj^es a number of well-marked extra- 

 vasations, some the size of a pin's head; on endocardium of left heart a 

 few, not larger than mere points. Lungs oidematous and of a pale red- 

 dish hue, especially marked along the edges of the lobes. Bronchial 

 .ylands enlarged, of a dark-red color throughout. The glands at the 

 root of the mesentery and about stomach very large and confluent, of a 

 mottled appearance. On section the medulla hyperaemic. Ou tearing 

 apart the coils of the large intestine the glands of the mesocolon ap- 

 l)eare<l as purplish red bean-shaped bodies gorged with dark blood. 

 Beneath the serous coat of the ciiicum at its very ti]j were numerous 

 l)unctiform extravasations. The kidneys were severely inflanied. Ou 

 tlic surface of both, numerous punctiforui extravasations. On section 

 the pyramids, including the tips of the ])apilla% of a dark red color. The 

 cortical jjortiou dotted with innumerable dai'k red i)oints. The lym- 

 ]»liatic glands in the abdomen itself were of the same purplish color. On 

 examining the ujucous membrane of the intestinal tract, a large, deeply 

 reddened patch of mucous membrane was found on the greater curva- 

 ture of the stomach. The small intestine seemed intact excepting near 

 the ileo-caical valve, where the longitudinal folds were of dusky red, 

 brought about by aggregations of minute dark-red points. A similar 

 condition prevailed" throughout the large intestine, giving the entire 

 surface a dark ai)i)earance. In many places small, blackish ecchymoses 

 indicated hemorrhages on the surface. The kidneys in this animal 

 seemed to have sull'ered most severely, next to the large intestine, which 

 in all our examination was obviously the seat of the severest lesions. 



Cover-glass preparations of the spleen, kidney, and liver, examined 

 immediately, rovoaled the same microbe which had bc«n introduced into 

 the system. 



Cultures in tubes of gelatine from blood of the heart and spleen re- 

 sembled precisely the pure cultures from previous cases. As usual, the 



