BUKEAU OF ANIMAL INDUSTRY. 495 



colonies in tlie culture of tlie blood were few and scattered ; those in 

 the spleen culture were innumerable in each needle track, (Plate II, 

 Fig. 3.) Two liquid cultures inoculated with blood from the heart and 

 one from the spleen were tested as above and found pure, containing 

 oidy the motile bacterium. 



The second pig (No. 112) inoculated with the same culture and in the 

 same manner as No. 114 died in the morning of December 12. The 

 temi)erature first rose to 105|o F. on December 8; diarrhea set in on 

 December 10. It was found dead on the morning of Decembei" 12. The 

 lesions closely resembled those of No, 114 with ilie following defer- 

 ences : The splcL'U was much enlarged, gorged with blood, and very 

 friable. On one border there were prominent red points, giving it a 

 ragged appearance. Nuuierous ecchymoses beneath tlni endocardium 

 of the lefc ventricle. On tlie surfac<.' of the lungs numerous dark-red 

 Sj)ots corresponding to hepatized lobules. These, about one-fourth of an 

 inch in diameter, were found throughout the lung tissue. The kidneys, 

 though congested, were not so seriously iitlected iis in No, 114, For 

 about 4 inches from the valve the summits of the folds of the mncosa 

 of ileum were deep red, consisting of aggregations of red points, A large 

 patch of mucosa in the ca'cum pur])lish. The large intestine, in gen- 

 eral, congested aiul coven d with dark -red points. No ulceration. The 

 enlargement of the lymphatics and intlammation of stomnch, as in pre- 

 ceding case, but more severe, the mucosa of the greater portion having 

 almost a black color, due to extravasation. Cover- glass i)reparations of 

 the spleen contained but few specimens of the bacterium of swine plague. 

 One cover-glass preparation, e:ich,of an inguinal gland, hei)atized lung, 

 blood from heart and liver negative. Two gelatine cultures, as well as 

 two liquid cultures, grew precisely as those of No, 114 and preceding 

 cases ; the liquids were found pure cultures of the njotile bacterium as 

 bei'ore. We had thus i)ro(luce(l tlie disease from liquid cultuies iu two 

 pigs and had obtained from each pure cultures of the bacterium which 

 had been introduced into the system. After inoculating N(»s, 112 and 

 114, a third healthy check-pig, No, HI, was placed in the same [len. 

 This animal remained well for two weeks after the death of the second 

 animal, when it began to show^ signs of disease. It died December 31, 

 uioro than a month after being penned with Nos. 112 and 114. The 

 autopsy demonsuated swine plague with extensive ulcerations of the 

 large intestine and implication of the lymphatic glands, luiigs, and 

 stomach, Jso bacteria weie found in three cover-glass [)re])aiations of 

 the spleen. This animal, thejcfore, lemained well uniil infected by the 

 two inoculated ones. It is evident that no check aitinia! will renudn 

 ultimately intact, owing to the manner of infection, and hence this must 

 not be looked for in experiments with swine plague, 



December IG twoguinea-])igs, female, were inoculated from the second 

 liquid culture of the s])leen, pig No. 114, No. 1 received i^*^" into each 

 thigh. No. 2 ^^'^ into one thigh. Both were sick on the following day, 

 crouching together and breathing heavily-. No. 1 died December 18, at 

 noon. No. 2 some time during the night. 



In both animals there was a considerable enlargement of the thighs, 

 inoculated, and of the surrounding parts. This was especmlly notice- 

 able in No. 2 when the inoculated side was compared with the other. 

 There was also a bluish discoloration of the skin extending to the vulva 

 and to the mammio. In both a sero-sauguineous effusion extended from 

 the place of inoculation in the subcutaneous tissue as far as the thorax. 

 The muscles of the thigh were infiltrated with blood. The vessels of 

 the skin were greatly distended, forming a deex^-red, arborescent net- 



