107 



bands only one appears, wliich is l)etween the two lines of Ory.-Hirmoglobin, and is 

 much broader than either of the bands mentioned above. This is the spectrum of 

 reduced Oiy.-H(rmoglobin or simply Hirmoglohin. 



METH^MOGLOBIN. 



The spectrum of Methivmoglobin is obtained by first preparing Oxy.-Hivmoglobin 

 crystals by treating dog's blood with ether and shaking it until it becomes laky, 

 then allowing it to stand in a cool place for an hour or so, at which time a firm 

 mass will be formed, due to the crystals. The mother liquor is separated from 

 the crystals by filtering through muslin or linen, squeezing the mass so as to ob- 

 tain the crystals in as pure a form as possible. The crystals are dissolved in dis- 

 tilled water and a dilute solution is examined with the spectroscope. The two 

 bands of Oxy.-Hcemoglobin appear. A few drops of potassium permanganate are 

 added and the solution gently warmed. If suflBcient time has elapsed for the ox- 

 idation of the Oxy.-Hctmoglobin, the two bands will have disappeared and instead a 

 single band in the red near the line ( ' between C and D. Nearly the entire spec- 

 trum is absorbed. Sometimes it is a little diflficult to get this band, but if the ox- 

 idation has taken place it will be seen. In the experiment at hand I left the so- 

 lution until the next day before it would give the above result. 



CARBOX-MOXOXIDE H.EMOGLOBIN. 



If coal gas be passed through blood which has been defibrinated, it will as- 

 sume a cherry-red color, the carbon-monoxide of the gas having driven off" the 

 oxygen of the Oxy.-Hamoglobin and taken its place. The reducing agents have no 

 influence upon this new substance, it being more stable than Oxy.-Hcemoglobin. 

 The two absorption bands are nearer to E than in the Oxy.-Hcemoglobin spectrum. 



H.EMATIN. 



The red corpuscles are composed of a proteid stroma and a brownish pigment 

 which is called hsematin. The iron is a part of the hsematin. It can be obtained 

 either as the acid ha?matin or the alkaline hfematin. 



In making the acid hiematin, I took 100 cc. of 95 per cent, alcohol and added 

 2 cc. of sulphuric acid, and then 10 cc. of blood; the mixture was boiled for about 

 an hour in a flask tube three or four feet long so that the vapor pa^ising off" would 

 be condensed in upper part of the tube and flow back into the flask. 



During this process a precipitate is formed which is acid hfematin. The so- 

 lution is filtered and the precipitate is dissolved in alcohol and then examined 



