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are white, slick, smooth surface, regular outline with a finely granular portion at 

 the edge. On Agar Streak it gives a cream-colored, raised convoluted growth 

 with folds running from the center to the edge. On Lactose Litmus Agar Plates 

 the colonies were large. They produce no change in the litmus, showing that lac 

 tic acid is not formed. 



FORM No. 2. 



Obtained from the air in the Purdue horse barn. The form is very abundant. 



Morphology. A micrococcus 1 to 1.5" in diameter. It often occurs in masses 

 of three or four. 



Biological Characters. A liquefying, motile ajrobic, micrococcus. In Bouillon 

 it produces turbidity and forms a yellow ring on tube at surfaces of the liquid. A 

 yellow precipitate collects in bottom of tube. On Potato it produces a raised, 

 yellow, granular growth along the streak. In Gelatine Stick culture a yellowish 

 growth occurs only on the surface which liquefies the gelatine. On Agar Plate the 

 colonies are of a brownish color, of irregular outline and of a granular appear- 

 ance. The colonies that grow on the surface are larger than those that grow on 

 the bottom of the plate. This form grows at a temperature of 102° F. ; giving a 

 medium, irregular, white slick growth along the streak. On Agar Streak it forms 

 a light yellowish, granular growth of irregular outline. On Lactose Litmus Agar 

 plates the colonies are large and white. They produce a small amount of lactic 

 acid. 



FORM No. 3. 



Obtained from the air in the Purdue cattle barn. Only a few colonies 

 obtained. 



Morphology. A bacillus 4" in length and about 1" in width. It occurs in 

 short chains. 



Biological Characters. A liquefying facultative anasrobic, motile bacillus. 



In Bouillon a white flacculent mass forms both on the surface and in the 

 liquid. In Gelatine Stick cultures it produces a slight white growth along the 

 puncture. It gives a light brownish, slick, luxuriant growth at a temperature of 

 102° F. It grows over the whole surface of the Agar and colors it brassy. On 

 Potato it gives a raised, wrinkled, luxuriant, white powdery growth. On Agar 

 Streak it forms a convoluted, raised, white growth, which is slightly granular at 

 the edge. The colonies on Agar Plate, white with a pale spot in the center 

 and are fringed at the edge. On Lactose Litmus Agar Plates the colonies are 

 ■white, raised, wrinkled, with a granular growth around the edge. The litmus is 

 changed slightly pink, showing a small production of lactic acid. The growth 

 on this media is quite rapid. 



