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Proceedings of Indiana Academy of Science 



appear and reappear and repeat this activity several times before they 

 may or may not become extremely numerous shortly before the death 

 of the animal. The organism may be first seen in the blood stream in 

 about two or more days and the extent of the disease may be from less 

 than two to many weeks. 



TABLE I. Showing the Results of Different Animals Infected with Nagana. 



S. C. — Subcutancou.s inoculation. 

 I. P. — Intraperitoneal. 



In the chronic infection the trypanosome appears as a rule several 

 days after the inoculation and then after running a very irregular 

 course for months, during which time only very few organisms are 

 usually observed, they may disappear entirely and the animal recovers. 



Technique of Studying TrypanosoineH in iJving Coudifion. — Since 

 the parasites are usually present in the blood stream the most convenient 

 method of obtaining blood is employed. When the organisms are nu- 

 merou.'^ in the blood a very small amount will suffice to reveal their 

 presence. 



