136 
BACILLUS 
AND CO. (0) ClOm CHE H N 
FERMENTATION FLUID* 
Y.| Smith’s Fluid, +2% Sucrose........... 67 a) none | none | 20.6 11-9 
Worn Same nacabOy Gy eee aes sco 61.8 .63 | none | none | 27.5 10.01 
21.2 
Y. Smith’s Fluid, +2% Glucose........... SS ala oso jnene none 36.5 
Y. Smith’s Fluid, no peptone, +2% Sucrose} 37.6 | 7.2 none | none 21.6 | 33.4 
SPs A Poe eee eae Riced aa aba 44.2] 1.9 none | none 33.6 10.2 
Y. Smith’s Fluid, + wort + .7% Acid...... 53.2 43) none | none | 31.7 14. 
ei SOUN UM Be MELE Metra nsa- ters cs octoateiae a rnets 45 .83 | none | none | 26.3 26.8 
Whol Sisbo ytd ea joa 00 0s Vipin ie ain Be AE hac 58.5 | none | none |........ 31.7 9.8 
: 
Ree IELT CHO MON LG eerste sjcicte #1: ise eels 43.2 | none | none |........ 40.7 | 16.2 
Y. Smith’s Fluid, +2% beet juice......... feet | Le 02) || MOneL ence 19.7 | 6.4 
X++. Smith’s Piuid Sa ein ae J Re waisich's Abeer ones 50 22 MONO i|)c cet: 32 | 18 
The method of systematic analysis in Hempel’s “Gas Analysis” was 
followed. The gas was transferred from the fermentation tube to a 
beaker inverted over water and from this to the measuring pipette. Five 
minutes was allowed for the absorption of the gases and another five 
minutes before the readings were made. Analyses were conducted where 
the temperature remained practically constant. The gas was passed into 
the solutions until no further diminution of volume was noticed. 
Carbon dioxide was absorbed by a solution of potassium hydroxide, 
oxygen by phosphorus and carbon monoxide by an ammoniacal solution of 
cuprous chloride. Hydrogen was estimated in two ways: (1) By absorp- 
tion by palladium and (2) by explosion with oxygen. When the latter 
method was used the gas, after explosion, was passed into potassium hy- 
droxide to make sure of the presence or absence of marsh gas in the 
* Fermentation fluid is the common one known as “ Smith’s’’ 10gr. peptone, 5 gr.sodium 
chloride, 1000 c. c. of water. The additions to this are marked in table. 
+Y. Bacillus separated from sugar beet by Miss Clara Cunningham. 
++ X. Bacillus separated from pear tree tissue by Miss Lillian Snyder. 
