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ful, and by this method a germ was obtained which I carried through 
a number of experiments and found to be in many respects like the germ 
described by Dr. Arthur in his History and Biology of Pear Blight. This 
germ was studied and kept alive until the latter part of May, when the 
pear was putting forth its new shoots, the young branches being then 
used for inoculation. Out of about ten inoculations, at different times, 
not one appeared to have any effect upon the tree. I concluded I was 
mistaken in the germ, which resulted in a second attempt to isolate the 
germ from the tree. 
By this time the trees were in all their foliage, thus making the dis- 
ease much more readily detected than previously. The same method was 
used as above described as successful, and out of six or seven attempts 
to transfer the germ from the host upon agar, five were successful. This 
germ thus obtained was then used to inoculate into the young growing 
shoots of the tree (Bartlett pear), and every inoculation that was made 
caused blight of the tree. This was during the month of June, the tem- 
perature being on an average of 70° F., the atmosphere moist, rainfall 
5.16 inches, being 1.84 above normal, thus favorable for the increase in 
growth. At the end of a week after the blight first began to appear it 
was not unusual to see the branches blighted 10 or 12 inches from the 
point of inoculation. 
In inoculation only perfectly healthy pear and apple trees were used, 
the former taking the disease with much more readiness than the latter. 
The germ was taken from streak cultures upon agar, a sterilized platinum 
needle being used to transfer the germ to the surface of the young 
twigs. After the surface had been smeared over with the germ, openings 
were made through the bark layers at this point, enabling the germ to 
get well started to grow before it might be washed off by rain. 
Leaves do not take the blight when inoculated. Naturally, the germ 
appears to be confined to the branches, the leaves only dying when their 
nourishment has been cut off. 
GENERAL CHARACTERS OF MICROCOCCUS AMYLOVORUS. 
The cells are oval, very little longer than broad, being about 
.59u to .8&. wide by .894 to 1.2 long. Cells are colorless, very refractive 
and difficult to stain, resembling spores in their relation to stain. The 
best results in staining were obtained with carbol fuchsine. When grown 
