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upon agar the cells are usually found single, very often in pairs and occa- 
sionally in chains of fours. When growirtg in bouillon or other nutritive 
wedia the germ exhibits independent movement, thus differing from the 
habits of the germ within the host. When sections of the diseased 
branches are placed under the microscope the bacteria show very little, if 
any, Movement. 
This germ appears to be aerobic, and increases in growth with an in- 
crease of temperature. 
Growth upon agar was most rapid during the months of July and 
August, the average temperatures for these months being 73.3° F. Later 
30 the season the germ ceased to grow with the same rapidity, although 
exposed to a high degree of temperature. 
Spore formation was not observed, and I am inclined to believe spores 
are not formed. 
CULTURES WITH LIQUID AND SOLID MEDIA. 
Cultures with bouillon after 48 hours at 30° C. remained perfectly 
clear, the growth all settling to the bottom of the tube. Not in any case 
have I observed zoogloea formed. 
In a pure corn-starch solution inoculated with the germ, the germ sur- 
vived and multiplied, but the starch was not chemically changed. Two 
cellulose solutions* made from Swedish filter paper were used. In one 
glucose was used and the other sugar was not added. In these solutions 
I planted the germ, and at the end of a week no apparent change had taken 
place in either solution, but the one which did not contain sugar originally 
was tested, and it was found that cellulose had been changed into 
glucose. 
Various fermentation fluids were used, but this germ does not ferment 
under any conditions. 
The most convenient solid media for the cultivation is agar. Colonies 
are white, slightly opalescent, when vigorously growing raising like beads 
from the surface. In a streak culture the outline is slightly feathery. 
Agar which had not been titrated, but used as made up, slightly acid, was 
found to give the best results in the growth of the germ. 
* Cellulose solution was made upas follows; Peptone, 10 grams; Salt,5 grams; Glucose, 
20 grams; Pure Cellulose, 20 grams; Water, 1000 C.C. 
