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In a stab culture in nutrient gelatine the growth spreads over the 
surface and along the line of inoculation, but does not penetrate the 
gelatine, thus remaining for several weeks without any sign of lique- 
faction. 
Probably the most characteristic result is the growth upon pear twigs. 
The end twigs to a length of two or three inches were cut from the 
tree. These were placed in water, the upper cut surface covered with 
the growing germ, and the vessels containing the twigs then placed under 
a bell jar for 24 hours. At the end of this time about two out of every 
four of the twigs had a beautiful growth over the cut surface. This 
growth was in the form of globules, as many as three or four globules 
being on one twig. These bead-like colonies, being white and raised from 
the surface, were perfectly apparent to the unaided eye. (See Fig. 1.) 
This growth increases, turning yellow when old, until entirely destroyed 
by moulds. 
Another very important characteristic of Micrococcus amylovorus is 
the manner of growth within the growing pear fruit. A half-sized Bart- 
lett pear upon the tree was inoculated in about the same manner as the 
branches had been. The pear ceased to grow and soon began to shrivel. 
At the end of two weeks the pear was removed from the tree, and upon 
examination the whole interior was found to be composed of a soft, milky 
substance, which appeared under the microscope to be made up entirely 
of bacteria. 
Half-ripe pears were taken from the tree, cut in slices, inoculated 
with the germ, and then placed under a bell jar. Colonies were found in 
24 hours, which resembled those already described upon agar. 
During the month of October cultures were taken from quinces which 
has been inoculated with M. amylovorus. This second germ proved to be 
entirely different from M. amylovorus, but alike in all respects to the germ 
separated from the tree in March. This germ, which I shall term No. 2, 
I feel convinced occurs within the tree. But what relation it bears to M. 
amylovorus, if any, I am not prepared to say. 
Germ No. 2 when transferred to pear twigs in water grows very well, 
but does not survive or grow with the same vigor as M. amylovorus does 
when placed under the same conditions. The growth of No. 2 is not raised 
in bead-like colonies, but grows in a continuous mass, over the surface, 
and instead of being white, is transparent. 
