WN 5yd) 
Microscopically the two germs are so near alike it is impossible to 
separate them. They bear about the same relation to stains and the cells 
are of about the same size. The arrangement of the cells of the two 
germs differ some, but this is not constant. 
CULTURES WITH LIQUID AND SOLID MEDIA. 
Cultures with bouillon after 24 hours at 30° C. are very turbid, and 
a thick pellicle is formed over the surface of the liquid. The germ grows 
well in a corn-starch solution, but the starch is not broken down. Cellu- 
lose solutions made as above stated were inoculated. At the end of two 
days both had fermented, thus in the one without sugar, sugar had been 
formed. In this one in which sugar was formed the gas production was 
very slow. 
Germ No. 2 planted in Smith solution causes fermentation and gas 
forms very rapidly. Mr. A. H. Bryan, a student of Purdue University, who 
was so kind as to analyze the gas produced by this germ, has given the 
following results of his analysis: 
From germ No. 2, separated from the pear tree in March, 1897: 
LOR NST nga Bea eee PEO SAG aes Pan maar 45.0 per cent. 
©). n.t8,c Bb 6 a0 ave DOES ale Witt Old cidio BrGpiOn Dione Damen ae .83 per cent. 
TRIPP ee Ae isin ania eo incor alate talatisr s eRe ete Aicve at byel ayo: seeieolists 26.3 per cent. 
QIET; “aS cl esid p10 Gr ceo agian DIM eter oreo ance 0.00 per cent. 
73.13 per cent. 
TReroa NAO KIe: re. INR ee soto A eeitio bn olobia bin tam oGolg 26.87 per cent. 
From germ No. 2, separated from the quince in October, 1897: 
CO ae gerne apt atre Near ghatatee arsine s Hela uustayS onalehs) spel ale vettesei ts 50 per cent. 
Oe ae i reee es nurstiel erro lasteara Douetireneirclota tovemattchetieLst sieicartr axe None. 
(XO ical Gad: Said eee eae eee Soot PS LN RE DRA crea MiNi ach coal gee None. 
1sL, done lee rolitobdle 5 Biolog o nla nea nod Gelso obemsoinieo dec mor 32 per cent. 
OLED We matics sy beers cent & Se pe Eee ERI ere: RoR Sesion mer eae 82 per-cent. 
TRYST HOA OVA POI LIN Gi praca Ric ROGGE Oe Dae oe Saari 18 per cent. 
It is presumed that the remainder in each case is nitrogen, but just 
what per cent. is nitrogen is not yet known. 
