Wal 
In order to determine if different shoots would give results similar to 
those observed in the elder shoot used in the experiment mentioned, a 
number of experiments were carried out last fall. The data, from all 
but three of these experiments, were very satisfactory and supported the 
first result. 
The three which were not very satisfactory were with the tomato, 
gladiolus and one of the maples. They will be mentioned later. In these 
experiments duplicates were carried out with all but the rose, dahlia and 
gladiolus; but were not carried out at the same time except for two of 
the experiments. The stems for the duplicates, while of the same species, 
were not always taken from the same plant from which the first stems 
were secured. The condition of the stems used was noted, efforts being 
made to secure the two as near alike as possible. The number and con- 
dition of the leaves were taken, but the amount of foliage present did not 
appear to haye any appreciable effect upon the results. If, however, a 
large surface of foliage was employed and suitable apparatus used, it 
would undoubtedly be apparent in the data. 
The length that the stems were decorticated was measured for each 
experiment, and the relation between the length of cortex removed and 
the time of wilting is shown in the table. 
In the first of the experiments performed last fall, stems from a ¢a- 
talpa were used. The tree stood in the open on high, dry, gravelly soil, 
and was about ten inches in diameter. Two stems each haying the same 
number of leaves were secured. From one the cortex was removed for 
about 2.8 cm. above the cut end, from which, just before it was put in 
the water, a fresh cross-section was made so as to expose fresh cells to 
the water. About 3 mm. were cut off so that there was left about 2.5 cm. 
of decorticated stem. A fresh cross-section was also made on the corti- 
cated before it was put in water. Both stems were put in water at the 
same time, September 20, at 11 a. m., with the air temperature at 18.5°C. 
The sky was cloudy and transpiration was slow that day, but by 9 a. m. 
the next day, with a clear sky and the temperature at 22.5°C., the corti- 
cated stem had wilted. The decorticated did not wilt until after five that 
evening. Six days later catalpa stems were tried again. This time the 
cortex was removed for only 1 cm. The stems, prepared as before, were 
placed in water at 7 a. m., temperature 21°C. At4p. m. that day, temper- 
ature 24.5°C., the corticated had wilted, the decorticated wilting about 
three hours later. 
