139 
ture was kept at room temperature, but at 30° C., gas is given off in 
twenty-four hours. 
In wort gelatine tubes the growth tapers from the surface along the 
needle track, having fine line of growth radiating from the main growth, 
then the gelatine gradually breaks down with the liquefaction. 
The colonies at first are rather thick in the center with filaments ra-. 
diating from the central mass. When liquefaction begins, which is inside 
of three days, the central mass breaks down and spreads as a sort of 
mycelial mass over the plate, resembling very strongly a mould growth 
when seen under the microscope; Ills. 6, 7, 8 show successive stages in the 
growth and liquefaction. 
The species is undoubtedly S. liquefaciens, as described in Saccardo," 
the cells showing the same variations and varying only slightly in size 
from.,that description. 
EXPLANATION OF ILLUSTRATIONS. 
Ten days’ growth in wort. x3820. 
bo 
Two weeks’ growth in wort gelatine. x450. 
8. Sixteen days’ growth in wort gelatine. x320. 
4. Twenty-four hours’ growth in wort in moist chamber. x334. 
5. Round cells from lactose solution in moist charmber. x320. 
6. Colony grown in wort gelatine, three days old. x30. 
7. Colony grown in wort gelatine, four days old. x35. 
8. Colony grown in wort gelatine, four days old. x35. 
1 Saccardo, P. A. Sylloge Fungorum, Vol. VIII, pp. 916-922. 
