141 
SACCHAROMYCES ANOMALUS HaNSEN(?) 
By KATHERINE E. GOLDEN, 
In his investigations upon spore formation in yeasts Hansen found 
three typical groups, these three differing either in the form of the spores 
or in their mode of germination. The third type is furnished by S. 
anomalus, the spores of which are hemispherical in form, having a pro- 
jecting rim around the flattened surface. The other two types are spheri- 
cal in form, but differ in their mode of germination. Hansen found this 
species in an impure Bavarian brewery yeast, and after he had called 
attention to the peculiar spores, they were also observed by Holm, 
Lindner and Will,* who likewise found them in impure brewery yeast. 
These investigators did not determine whether the peculiar spores which 
they observed belonged to Hansen’s 8. anomalus or to allied species. Bay? 
gives the habitat as impure brewery yeast and grapes,.as does Kayser.® 
Hansen’s* description of the yeast is very brief, as is also the descrip- 
tion by J6rgensen’ and that given in Saccardo" so that the form of the 
spores is the most characteristic feature. 
A yeast which develops the same form of spores, I found associated 
with another yeast on the skin of lemon. This is a new habitat for the 
yeast, and I can find no mention of any such yeast occurring in this 
country. 
The cells are round or oval, occurring either singly or in pairs, though 
occasionally in a vigorous growth in wort colonies consisting of many 
cells are found (photograph 1). ‘The cells are very small, measuring 2.4 
# in breadth by 3.3 “ in length for the small oval ones to 6.6 jm in 
diameter for the cells containing spores (seen in photograph 2). 
In wort gelatine plate cultures the colonies are of a dull, grayish white 
color, round, with rather even outline. They grow rather slowly under 
the best conditions. The photograph (8) shows a _ forty-eight-hours’ 
growth. The wort gelatine plates seem to offer better conditions for the. 
1 Jorgensen, A. Micro-Organisms and Fermentation, 1893, p. 182. 
2Bay,J.C. Am. Naturalist, Vol. XX VII, 1893, pp. 685-696, 
3 Kayser, E. Les Levures, p. 104. 
4Hansen, E.C. Central. f. Bakt., Bd. XIII, 1893, pp. 101-102. 
5L.c., pp. 181-182. 
®Saccardo, P. A. Sylloge Fungorum, Vol. VIII, pp. 216-228. 
