142 
formation of spores than any of the other cultures, spores being found in 
four days at room temperature. 
In both wort and beef broth gelatine tube cultures the growth is 
practically the same. The growth has ‘a dry appearance and forms a 
dense mass at the needle puncture, without any tendency to spread. The 
growth tapers gradually from the surface along the needle track until 
at the bottom of the tube it is just perceptible. It has a characteristic 
crinkly appearance along its whole length. 
Cultures in Pasteur solution, with 5 per cent. sucrose, lactose and 
dextrose, were made in fermentation tubes, and also in wort which con- 
tains maltose. In sucrose no fermentation occurred; there was a heavy 
growth, however, which caused a strong turbidity of the liquid in the 
bulb and a heavy sediment; the liquid in the tube remained clear. A film 
was formed which extended up the sides of the tube. After five days 
no spores were found either in the film nor in the sediment. Only 
a very slight growth occurred in the lactose solution, this forming 
a delicate film anda slight sediment. No gas was formed. The cells 
in lactose occur singly or in pairs and appear poorly nurtured (photo- 
graph 4). In the dextrose solution the growth was vigorous, forming a 
heavy sediment. Fermentation commenced in four days, and in twenty- 
four hours 5 ce. of gas were formed. In this, as in the sucrose solution, 
a strong film was formed. No spores were found in the film nor in the 
sediment even after seven days’ growth. In the wort the fermentation 
ras much more vigorous than in the dextrose, 10 ce. of gas being formed 
in the time that only 5 cc. were formed in the dextrose solution. Even 
before fermentation ceased a film formed in spots on the surface. In the 
wort a delicate ethereal odor is generated, which is very pleasant. Spores 
were just beginning to form in the film and sediment in eleven days. 
Spores formed more readily in a wort gelatine plate culture than even 
in the regulation manner on a gypsum block. In the plate they formed 
invariably in four days, not to any great extent, but sufficient to be found 
in every microscopic examination. The cells in which the spores form are 
large, and just before the formation the protoplasm becomes very granu- 
lar and refractive. As the culture ages and more spores are formed they 
are found free from the cell wall and in groups ranging from two to four- 
teen in number. Spores can be seen in photographs 5 and 6. 
It is not quite certain whether this is the same species as Hansen’s 8. 
