288 Proceedings of Indiana Academy of Science 
TABLE 3 
Series III East—Rotation Continuous Wheat 
Plot 1 Unlimed Plot 2 Limed 
Nitrates—Parts per million Nitrates—Parts per million 
Month Moisture % Moisture % |——————__- 
Fresh Incubated Fresh Incubated 
Samples Samples Samples Samples 
21.5 28.51 40.73 22.4 26.80 43.29 
18.35 21.55 50.88 21.0 4.05 36.48 
15.48 7.56 26.46 16.15 11.42 26.66 
10.65 14.30 3.57 9.01 7.03 31.638 
12.23 10.93 21.85 13.17 11.05 33.14 
14.50 11.21 17.92 15.88 11.40 15.99 
15.28 16.98 22.68 13.03 22.47 33.10 
23.16 6.24 20.82 24.37 8.46 19.03 
Total per period.......... 117.28 204.91 102.28 239.27 
Ave. per month.......... 14.66 25.61 12.78 29.90 
The content of the fresh samples for the unlimed plot was high 
in March, April, and fairly high in June, July, August and September, 
while that of the limed plots was high in March and September, and 
fairly high in June, July, and August. 
The incubated samples gave an average of 25.61 parts for the un- 
limed and 29.90 parts for the limed. Here again the samples taken 
during the growing season contained the most nitrates. 
The addition of lime to the soil brings about conditions more 
favorable to plant growth and bacterial activities. This in all prob- 
ability accounts for the higher averages of the treated and untreated 
samples of the limed plots compared with the unlimed plots. The limed 
wheat no doubt made greater growth during the months of April, May 
and June than did the unlimed wheat, and therefore the fresh samples 
showed a lower average than those of the unlimed wheat. 
TABLE 4 
Series I West—Rotation Corn, Oats, Wheat, Soybeans 
Plot 1 Oats Plot 2 Wheat 
Nitrates—Parts per million Nitrates—Parts per million 
Month Moisture % -— Moisture % |— 
Fresh Incubated Fresh Incubated 
Samples Samples Samples Samples 
Mars. os 6s6s OSG AEE E 21.8 20.44 39.87 22.3 28.80 49.18 
ANT cama Be AW Ae 20.60 14.10 37.45 22.2 12.32 36.96 
Mag fe We © aceite 16.55 3.83 21.07 16.12 7.61 28.47 
SURES. cee ek ee aioe. 12.61 1.83 21.96 16.09 3.81 30.47 
aby Pose eee 14.90 11.28 22.56 13.14 11.05 25.78 
UES tee ee cee nia yee orate 18.07 7.81 9.96 17.78 7.78 15.56 
Santina ts eee oe hee : 11.79 9.05 21.76 12.61 5.48 10.98 
Ootic Se end cee eeae 22.17 6.16 12.33 22.74 4.14 8.28 
Total per period.......... 74.50 186.96 80.99 205.68 
Ave per month.......... 9.31 23.37 10.12 25.71 
DISCUSSION OF TABLE 4. 
Series I West—Rotation Corn, Oats, Wheat and Soybeans. 
The average of the nitrate content of the fresh samples for the 
period was 11.92 parts per million parts of moisture-free soil for the 
