THE CULTIVATION OF TRYPANOSOMA BRUCEI. 



269 



By means of bulb pipettes the desired constituents of the blood may be 

 removed. 



Shortly before use the desired number of agar tubes were melted in the 

 water-bath, cooled to 60° C. and two volumes of defibrinated rabbit blood 

 minus the white blood cell layer were added. The mixture was thoroughly 



-serum 



-film of white bloo^i cells 



red blood cells 



Figure V — Centrifuge tube. Full Size. 



agitated by rolling in the hands in an upright position, by tapping on the tube, 

 or by employing both methods. This well mixed medium was alloAved to 

 solidify in an incline position. It was found advisable to employ the blood 



84.32 — 18 



