278 PllOCEEDINGS OF THE INDIANA ACADEMY OF SCIENCE. • 



for use. Xo list is made here of the chemical apparatus that is necessary for 

 nitrogen, moisture, nitrates or other chemical determinations that are to be 

 run on each sample. 



List of Apparatu.s Needed Before Plating is Finished 



10 Bacteriologist's soil samplers capped, plugged and sterilized. 

 1 Driving mallet. 



1 Driving head. 



1 Light wooden mallet. 



1 2 quart sauce pan. 



1 Aluminum spoon (tablespoon .size.) 



11 12 ounce plugged and sterile salt mouth bottles.* 

 55 8 ounce plugged and sterile salt mouth bottles. 



10 16 ounce salt mouth bottles with rubber stoppers. 

 30 One half pint jell glasses (sterile.) 



1 Set Balances sensitive to 0.1 gram with a capacity of 400 grams per 



pan. 

 (S Liters of sterile water. 



11 Rubber stoppers to fit 12 ounce bottles. 

 72 Test tubes of sterile media. 



67 Sterile 10 ec. vol. short form pipettes, plugged and in eases. 

 24 Sterile 1 ee. vol. short form pipettes plugged and in cases. 



1 Sterile 5 cc. pipette plugged and in a test tul)e case. 

 72 Sterile 100 millimeter petri dishes. 

 10 One pint Mason jars with rubbers. 



1 200 cc. graduated cylinder, plugged and sterilized. 



1 100 cc. graduated cylinder, plugged and sterilized. 



2 (i-inch Battery jars. 



The numbers given are exactly what is needed. More apparatus is always 

 prepared to take care of mishaps or mistakes. 



Procedure 



Take 10 samplers, remove the caps, rub a piece of paraffin over the out- 

 side of each just above the cutting edge (this is so the cap will slip on easily 

 after the sample is taken), and replace the caps on the samplers. The cap 

 and the sampler it fits should be stamped with the .same number. Plug the 

 samplers with absorbent cotton, sterilize them in hot air oven, cool and stand 

 in a suitcase. Keep the .samples upright. 



Plug, with absorbent cotton, the .55 eight ounce bottles two hundred cc. 

 graduated cylinder and one hundred cc. graduated cylinder, place them in 

 one of the made over baking ovens and sterilize them. Plug the 11 twelve 

 ounce bottles with absorbent cotton, weigh each to the nearest decigram, 



♦See note at I'lid of article. 



