298 PROCEEDINGS OF THE INDIANA ACADEMY OF SCIENCE. 



opposite pan of the balance. 99 ce or 90 cc aliquots of distilled water were 

 measured out by means of a 100 ee graduated cylinder and poured into 

 each bottle. In no ease was the amount of water poured in more than .35 

 of a gram away from that desired. Water was taken out or added so that 

 each bottle contained the weight desired. 



The bottles were sterilized for 15 minutes under 18 pounds pressure of 

 live steam and then the pressure was reduced at the rate of one pound per 

 minute; the door being opened 35 minutes after it was first closed. 



Test 1. 



Two eight ounce salt mouth ])ottles containing 99 grams of water and tw<> 

 containing 90 grams of water. 



All four bottles were plugged with absorbent cotton. 

 The results of this test are given in Table VII. 



Table VII. 



Test 2. 



Foiu-teen 8 ounce salt mouth bottles containing 90 grams of water. 



Seven were plugged with absorbent cotton and seven were left unplugged. 

 They were set in the autoclave in sees of two. The two at the rear were num- 

 bered 1 and 2, and the two nearest the door 13 and 14. Even numbers denote 

 bottles having no plugs. E.xperiment conducted as Test 1. Results are given 

 in Table VIII. 



