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were left in this environment for various lengths of time ranging from 

 one week to one year. At the end of these respective periods the small 

 dish with the mucor within it was removed, and with the utmost care 

 a piece of moist, sterilized bread was introduced, after which it was 

 set aside in a warm, dark place. 



In all cases but the last one a vigorous growth was made within 

 seventy-two (72) hours and in many cases zj^gotes were found within 

 a week. 



The results of the cultures remaining in the desiccator for on(! 

 year were not very conclusive, due to a slight accident. The culture 

 of the zygotes, that was freshly matured when it was placed in the 

 desiccator, produced growth within twenty-four (24) hours, and sporan- 

 gia within forty-eight (48) hours, but the culture with the older zygotes 

 in it failed to grow within two (2) weeks after being removed from 

 the desiccator and moistened, though upon further moistening a vigorous 

 growth was produced. Unfortunately, though, when the culture was 

 being moistened the second time the lid slid off for an instant and there 

 is a slight possibility of spores from the outside gaining entrance. 



In one of the first experiments performed, growth failed to take 

 place until further moistening, and it is the belief of the author that 

 such was the case in this last experiment. 



2. Resistance to Heat of Zygotes in Desiccator. — Zygotes were 

 placed upon oven-dried bread, put in a sulphuric acid desiccator, and 

 then placed in sm. oven at GO degrees centrigrade for various lengths of 

 time, ranging from seventy-two (72) hours to five (5) weeks. At the 

 end of these periods a culture would be removed and the bread moistened 

 with sterile water. In all the cultures the zygotes survived the heat, 

 and within forty-eight (48) hours after being removed there was a 

 vigorous growth, in many cases zygotes being formed within a week. 



Another set of experiments was run along similar lines, through 

 in this case the temperature was raised to seventy (70) degrees centi- 

 gi'ade, the time ranging from one week to one month. In the case of 

 the freshly matured zygotes, or as will hereafter be termed Neiv Zygotes, 

 a culture was able to survive two (2) weeks of heat and desiccation, 

 though at the end of three (3) weeks, no growth took place when 

 placed in favorable environment. The one year old zygotes were not 



