184 



ing small cubes of bread, placing one in each test tube, sterilizing them, 

 and then inoculating the bread; after which the test tubes were tightly- 

 plugged and placed in warm water the temperature of which ranged 

 from forty-five (45) to seventy (70) degrees centigrade. 



The following table will give the temperature and the longest time 

 for each of these temperatures that the zygotes were able to remain 

 in it, and still retain the power of germination. 



TABLE I. 



*Experiments of longer duration were not made for this temperature. 



6. Resistance of Zygotes to Various Chemicals. — The resistance 

 of the zygote and the growing mycelium toward a few chemicals was 

 tested out. Molecular solutions of NaCl (common salt), Fe:;CL 12HaO, 

 CuSOj, and C1.H..OH (ethyl alcohol) were the solutions used and were 

 the only moisture that the germinating zygotes and growing mycelia 

 received. Oven-dried bread was moistened with the chemical and then 

 inoculated with zygotes after which the cultures were set aside in a 

 warm, dark place to germinate. The first column of Table II indi- 

 cates the highest molecular solution, or fraction of molecular solution, 

 in which the zygotes and the mycelia would grow; while the second 

 column shows the same in terms of per cent of the chemical in solution. 

 Column three gives the highest molecular solution in which a vigorous 

 growth took place, the last column indicating the same thing in per 

 cent of the chemical in solution. 



TABLE IL 



