ibllowed by a cell division (fig. 7), but afterwards each of 

 the two daughter nuclei divides again. The actual process 

 of division I hâve not observed, but hâve only found four 

 nuclei; the second division evidently talées place very 

 rapidly, for I hâve looked through hundreds of préparations 

 of about this âge, without getting the actual stage of 

 division with the exception of the case represented in fig. 7 

 where the upper nucleus shows a prophase stage. This 

 second division takes place in such a manner, that the 

 axes of division are perpendicular to each other; for the 

 upper pair of nuclei the axis is at right angles to the 

 lenght of the embryo-sac, and for the lower pair it is 

 parallel to it. 



Before this last division has taken place, the embryo-sac 

 is still seen to be a single cell, as was already stated 

 above; after this division four cells, each with its nucleus, 

 may be observed. It is of course possible, since I hâve 

 not seen the actual nuclear division, that the latter is 

 preceded by a cell-di vision, in such a way, that each cell 

 contains a nucleus, and that afterwards each of thèse 

 two cells divides again, after its nucleus has divided. Only the 

 case mentioned above (fig. 7) makes this very improbable. 

 However this may be, there are finally four cells, which, it 

 should further be noticed, are not separated by cell-walls — 

 four naked protoplasts therefore (fig. 8, 14). Of thèse four 

 two, the synergids, lie at the top, next to each other ; then 

 follow the other two, one under the other, the upper one 

 of the pair being the egg and the lower one ail that 

 remains of the embryo-sac with the upper polar nucleus. 



Considering this lower cell first, we observe, that it 

 romains small and that pretty soon its nucleus clumps 

 to a little bail of chromatin, in which structure can no 

 longer be discerned (fig. 9); often the antipodal nucleus 

 may be seen at the same time. In other cases no remnants 



