155 
moved, partly dried hy standing in absolute alcohol, filtered and dried in 
an oven at 105° C. Duplicate Kjeldahl nitrogen determinations were made 
on each of the respective precipitates. Each of the six duplicates were 
found to check quite closely, thus indicating that there was nothing gained 
by the continued solution and precipitation of the mucoid. Incidentally 
it might be mentioned that there was mucoid lost at each precipitation by 
incomplete precipitation. This was evident from the fact that by most 
eareful work, starting with 20 grams mucoid, it was only possible to wind 
up with about 13 grams actual dry mucoid. 
In conclusion, it may be stated that tendon mucoid coagulates, the 
amount increasing with the duration of boiling, in the presence of neutral 
salts; that mucoid is not completely precipitated by an excess of dilute 
acid; that in a mixture of albumin and mucoid, most of the mucoid and 
part of the albumin are precipitated by dilute hydrochloric acid in excess. 
As for a remdy, nothing is offered as yet, but this work seems to show 
that the older methods are inaccurate. 
This work was carried on largely during the last year in the labora- 
tories of the College of Physicians and Surgeons, Columbia University, 
New York, where the author was associated with Dr. William J. Gies, and 
under whose supervision the details were worked out. Owing to the non- 
possession of the actual notes at present, many valuable data must be left 
out of this paper, but every statement made can be further demonstrated 
by experimental data. 
