quantity of sediment and forms than in the instance where one hour and 
twenty-five minutes was required for filtration. Some permanent slides 
were prepared in 1896 and part of these I still have, which show some of 
the various Algze forms obtained by the method above referred to. These 
slides were prepared by making a mounting fluid of the following sub- 
stances : 
AN COMOU OD Vatak citoucichaas od aiersuc oe orehaealy ans 10 cem. 
AGI CETM Ee cneesrspeucee aie ese eco temecsuser sa epenereoronorerels Senos 
IO en Kae PW eS woe aoa ciao alot GOO Or Bie ss 
ENCOUN CAC Oe iey o giateasacrst tiotssemocs: ore re: shapeuea cus cnene 30) > f 
Specimens mounted in this mixture should be sealed with balsam. 
The slide should be thoroughly cleansed and dry before ringing the cover- 
glass with balsam. The only danger from the loss of specimens so mounted 
is from the liability of the balsam to crack and allow the liquid under the 
cover-glass to evaporate. For this reason they had better have the balsam 
covered with a layer of dammar-lac or shellac, and be noticed from time 
to time and not kept in too warm a place. 
Dilute glycerin seems also to be a good medium for mounting Algie. 
One specimen of Pandorina has been preserved and mounted in it fifteen 
years and is still apparently as green and as perfect as the day it was 
mounted. Camphor water’ and glycerin also seem to give good results 
from the standpoint of preservation. 
Other forms of the Algwe of this list not found in the water works 
reservoir have been observed at different times and recorded as found. 
It is not supposed that this list of Algze here given is by any means com- 
plete, but gives an idea of a few out of an enormous number of forms that 
must be widely distributed. A good many of the forms here mentioned 
have been found by Mr. A. B. Williamson, one of the students in the Botany 
Department, and reported to me for the following list. 
A list of the growing forms of plants in any locality is best made and 
more complete when extended over a series of years, so as to include those 
individuals which for various reasons or changes of conditions do not ap- 
pear during one season. 
! Stokes—Analytical Keys to the Genera and Species of the Fresh-water Alge, 
p20: 
