392 
chambers had a few drops of the culture medium placed in the bottom, 
so as to keep the vapor tension unaltered. 
The cultures were examined at regular intervals, as indicated in the 
tables, those in the flasks having specimens taken for examination with 
the miscroscope. The points noted were the swelling of the spores pre- 
ceding germination, the length of hyphie, and the earliest appearance of 
conidiophores, for the cultures in the moist chambers. For all other cul- 
tures a hand lens was used to determine the first appearance of germina- 
tion. The appearance of the conidia was shown by the blue color, and the 
maturing by the change in color from the blue to green, and then to olive. 
The volume of mycelium and conidia was noted to determine the extent 
of development. 
PENICILLIUM GROWN IN TOMATO BOUILLON 500 cc. 70° F. 
Per Cent Time to 
Seal ete Germ. Development. 
Hours. 
_— 24 Spores germinated. 
48 hours—surface covered. 
72 hours—spores developed, surface blue. Hyph uniform in outline, protoplasm 
homogeneous, many vacuoles. 
120 hours—fully matured. 
1-12 48 Thin ring at edge, small colonies submerged. 
120 hours—surface covered, blue. Hyphw, uneven outlines, protoplasm granular, 
walls broken easily 
240 hours—fully matured. 
1-10 48 Slightly less developed than in the preceding, otherwise alike. 
240 hours—fully matured. 
1-5 120 Thin interrupted ring at edge. 
168 hours—spores swollen, irregular in outline, filled with coarsely granular pro- 
toplasm, walls broken by cover glass. 
336 hours—surface dotted with colonies, showing blue spots. 
348 hours—fully matured. 
1-2 —~— 
The effect of the sodium benzoate on the development is shown in a 
retarded and abnormal development. these being accentuated as the amount 
of the salt was increased, to a point where no development occurred. 
