393 
PENICILLIUM GROWN IN 10% Tomato GELATIN. 100 cc. 70° F. 
Per Cent utes 
Shdl, eae Germ. Development. 
' Hours. 
— 24 White colonies dotting surface. 
96 hours—surface covered, green, mature. 
1-12 24 Same as control. 
1-10 24 Same as control. 
18 24 Same as control. 
1-6 24 Less developed than control. 
96 hours—surface covered, nearly all green. 
432 hours—mycelium curled up round edges. 
1-4 48 Small white colonies dotting surface. 
96 hours—surface covered, nearly all green. 
342 hours—mycelium curled from edges to center. 
1-2 72 Small white colonies dotting surface. 
96 hours—surface about two-thirds covered, center green. 
342 hours—mycelium curled up so as to enclose the spores. 
In this experiment in which a solid medium was used, the effect of 
the sodium benzoate on the development of the mould was not marked, 
except in the cultures containing the larger amounts. In these there was 
a slight retardation, and also a curling up by the mycelium from the sub- 
stratum. 
PENICILLIUM GROWN IN TOMATO BOUILLON IN Motst CHAMBERS, 70° F. 
Per Cent LMS 
SEAL [sea Germ. Development. 
Hours. 
== 24 Short tubes formed. 
48 hours—well developed colonies forme 1. 
1-12 24 Short tubes formed. 
48 hours—colonies smaller than in the control. 
1-10 24 Tubes just forming. 
48 hours—less development than in the 1-12th solution. 
1-8 24 Less than in the 1-10th solution. 
48 hours—less than in the 1-10th solution. 
1-6 48 Spores germinate1, shorter tubes than in the 1-Sth solution. 
1-2 96 Spores germinated, short tubes. 
Nove.—In 120 hours the control was exhausted, having empty hyphe; the 
other cultures, with the exception of the 4% solution, have hyphe with many 
vacuoles in the protoplasm, the conidiophores formed are apparently normal. 
