B94 
The effect of the antiseptic on the development of the mould grown 
in the moist chambers was not so pronounced as when a larger quantity 
of solution was used. Neither was the effect always uniform; sometimes 
the spores in the 4% and the 4% solutions merely swelled, but no develop- 
ment of hyphz occurred; in others short tubes developed from some of 
the spores, while still other spores showed no changes whatever. 
To test the effect of the larger quantity of solution, inoculations were 
made into flasks containing 100 and 500 cc., respectively, of the solutions. 
The results indicated that the effect of the antiseptic on the mould de- 
velopment was greater when grown in the larger quantity of the solution. 
PENICILLIUM GROWN IN TOMATO PULP, IN PETRI DISHES, 65° F. 
Time to 
Per Cent G 
Sod. Beny. ae Development. 
s = 
-— 72 White colonies dotting surface. 
96 hours—spores formed. 
192 hours—surface covered, green. 
1-12 144 White colonies growing up on side. 
192 hours—spores formed on one side, colonies starting in center. 
1-10 192 Colonies started in center. 
312 hours—spores formed. 
1-8 | 144 White colonies growing up one side. 
192 hours—spores formed. 
6 | — - - —— 
1-4 — | -- 
© ne ee 
The pulp used in the experiments was of fine quality, and without 
any added ingredients such as are used in ketchup, and was used so as to 
determine the action of the sodium benzoate alone in the pulp. During 
the early stages of development, the mould grows down into the pulp, so 
that the whole surface of the hyph:e acts as an absorbent and would thus 
be affected to a greater extent than where only a part of the surface was 
in contact. This may serve to explain the more pronounced action of the 
sodium benzoate when in the pulp, and also the fact that after the mould 
has developed sufficiently to grow out of the pulp the development becomes 
more nearly normal. 
