396 4 a 
had 200 ce. water added, then stood for 12 hours, after which the juice 
was filtered. 
3. This was similar to 2, but the vessel was covered closely during 
the steaming. 
For the experiments, 50 cc. of the filtrate from each set were placed 
in flasks. They were inoculated with the mould without any previous 
sterilization. The following table shows the time required for, and the 
effect on, development : 
PENICILLIUM GROWN IN CRANBERRY JUICE. 
s 
Days to 
Mepium. Germinate. Development. 
Rawitiices. toe cenn5. See ore 4 | Short tubes. 
7 days—only small white colonies. 
. | € . 
Juice cooked, open. . 2 Short tubes. 
7 days—colony green, 
Juice cooked, closed 2 | Surface nearly covered, white. 
| 
7 days—surface green. 
Raw juice+ 10cc. water. . ‘ 3 Small white colony. 
| 7 days—surface green. 
| 
Juice cooked, open + 10ce. water 2 Surface nearly covered. 
| 7 days—surface green. 4 
[> > 
Juice cooked, closed + 10cc. water 2 | Surface nearly covered. 
; | 7 days—surface green, 
| 
After two weeks’ development, the color of the spores of Penicillium 
was a yellowish green, instead of the normal bluish green, and the my- 
celium was very scantily developed. ‘The surface had a somewhat granu- 
lar appearance, instead of the smooth, even appearance of a normal culture. 
The filaments, when seen with the microscope, were thin, shrunken, and 
clear, with distorted outlines. The cultures were kept for months, re- 
maining scanty and granular looking, and a peculiar feature was that no 
development of bacteria occurred, even in the uninoculated ones, though 
no sterilization had been done, and the uninoculated were exposed to the 
