198 
By the Reichert-Meissl process, we obtain only a certain fraction of the 
total volatile acids and which is fairly constant if carried out under stand- 
ard methods. 
To determine the relation of the volatile acids obtained from the R.- 
M. process of distillation to the total volatile acids, distillation was made 
with steam. By this means it is possible to estimate the total volatile 
acids. The usual method of saponification and precautions were taken as 
in the RM. process. 
One thousand ¢.c. were distilled with steam and an alignment por- 
tion titrated which gave a total of 6.03 per cent. volatile acid as butyrie 
acid. In the R. M. process, 5.19 per cent. of acid was obtained from the 
same butter fat. Thus we see that only 86 per cent. of the total per cent. 
of volatile acids were obtained by the R. M. process. 
The Rate at which the Volatile Acids Distill by Means of Steam. 
The same methed was used as in the previous experiment in determin- 
ing the total volatile acids. ‘The distillation was collected in portions of 
50 ¢«.c. and titrated with — NaOH. Ywenty fractions were titrated and 
10 
the result shown in Table II. 
RARE) wile 
No.. < a Fe He al 2. 3 4. Dien | eit lari 8. | 9%. |, LOC ide 
C. C_—Na0H .|12.7 | 8.48 5.53) 3.66)2.4 1.52, 1.00} .70) .60} .45] .30 
Per Ped vol. acid as butyric ») 03, 1 36 89 59) .39|  .25] za -11) .08) = .06) .047 
NOs eee Sie Aina AS ea 12 js) (PES Los be ur | 18. |} 19. | 20. Total 
C. C.—Na0H.... ..| 30} 25 | 29 | 20} 18 | 15] 14] .13| 20] 38.74 
Faveant ¥oh acid as butyric... . ...| .047) .04 |) .03 | .03 | 028] .023) .022) .021| .017 6, 22 
The weight of butter fat taken for the experiment as shown in Table 
Il was 5.50 grams. The per cent. of volatile acid and distillate is based 
on five grams of fat. 
From Table II it is seen that the first fractions contained the greater 
part of the volatile acids, decreasing rapidly after the second fraction, and 
that the volatile acids are practically all distilled when 1000 cc. have 
been collected. 
