94 
25 transters tovsoil ext. (awboclaved)) i... sie nies caret a eapeiete rene lteter 20 made growth. 
A>ibranstersitO-ACAar AONE sot sts heredeheak tece She Featss ae sues ole weet eee eleee everett 20 made growth. 
2 SIUrAMSLECS MOPS ALY ATG TSO Mec susteye tay oieee serous fe ewes comtakie amie cere lie teem dee « 19 made growth. 
Notes. 
When’ tubes of organisms grown originally on same media were put side 
by side the following differences were noted. 
(1) Agar alone supported very poor growths. 
(2) Agar and soil supported fully as poor growths as agar alone. 
(3) The two extracts acted about the same, although the heated extract 
grew the organisms originally grown on Na. asp. agar a little the best. 
(4) L. and B. agar and Na. asp. agar supported good growths. 
(5) From any macroscopic test the growths on the L. and B. agar were 
far superior to those on the Na. asp. agar. 
Tose Mepia Test II. 
Samples of 6/14, 1915. 
Samples from Tree VI—24. Plot C. 
Laurel. 
One ee. portions of the 1-400,000 dilution of the sample were plated on 
the following media: 
Lipman and Brown agar. 
Conn’s sodium asparaginate agar. 
Agar alone. 
Soil and agar (Purdue soil). 
Soil extract (unheated) and agar. 
Soil extract (autoclaved) and agar. 
(15 gms. agar in all media.) 
Transfers were made from best colonies on each media to slants of other 
media. Tables give results of growth on these agar slants at end of 5 and 
14 days’ incubation at 22° C. 
