96 
Notes. 
When tubes of organisms grown originally on same media were put side 
by side the following differences were noted: 
(1) Agar alone supported very poor growths. 
(2) Agar and soil supported fully as poor growths as agar alone. 
(3) The two extracts acted about the same, although the heated extract 
grew the organisms originally grown on Na. asp. agar a little the best. 
(4) L. and B. agar and Na. asp. agar supported good growths. 
(5) From any macroscopic test the growths on the L. and B. agar were 
far superior to those on the Na. asp. agar. 
Tuse Mepia Test III. 
Samples of 6/25, 1915. 
Sample No. 6. Rye Plot. 
Cover Crop Investigations. 
One ee. portions of the 1 to 400,000 dilution of this sample were plated 
on the following media: 
Lipman and Brown agar. 
Conn’s sodium asparaginate agar. 
Agar alone. 
Soil and agar (Purdue soil). 
Soil extract (unheated) and agar. 
Soil extract (autoclaved) and agar. 
(15 gms. agar in all media.) 
Colonies developing well on first two media listed were put on other 
media and growth noted at end of 5, 11, and 15 days’ ineubation at 22% C, 
From 4 Colonies on L and B agar to 
5 Days. 11 Days. | 45 Days. 
as = = | = 
: 3 g. | 4 g. 4g. 
AAS DCA errs care nie wes ape nae ; 
1 
2g. org. 3g. 
Soiuloxt: Moimneahed) iy. = occ. ts 2. ssterer eee ‘ 
2— 2 1 — 
| 
é 3g 32. 3¢g 
Led Felis aie: ek yd SOG Eo ln el or ee eS 
1 = 1 — 1 — 
