From 4 Colonies on Na. asp. agar to 
: — —— = 
5 Days. 11 Days. 15 Days. 
MANOR AR AD a tkhecdie acu a ate BS ea a 4g. 4g. 4g. 
: (2 g 3 2. 4g. 
SoOLsexos (UMMeAtTCG): act. ccs. ns cece esc ee lo oy ee 
) 8 g 3 g. 3g 
LETITIA (CEE ARE ater a a sei cra Ae 
i i 1 — 
Summary 5 Days. 
ABE MSLeLUSAuOUls AT Gps Lae Aly. toes cho sole oe theme ae se ePeeue nine sees s 4 made growth. 
Manan Ser snuOl Nanas Dara saleta einen s, Paminis na erate Saris oe oo made erowth: 
Secransters: to) soil ext.) (umheated |)... = is... a6 «hes ees eo ss ese Sue 4 made growth. 
SEURAIISEEESULOmAP Alea OMG sacs crag a fate cusuc ac) Sse ate, cs = Sane Boars ote 6 made growth. 
Summary 15 Days. 
PALA STOrs sO meal Cas a@al aces «octal eae. aad kale ates todeoe ora Gina ere aan 4 made growth. 
MBEAN STeLSRUOM Na HAS Da Sal nek eae cece eee teione ots = se ieiee iA ronanencht 4 made growth. 
Saran stersslOus Olle xs (Umea ted))itace., suns etelotes oe sted Nevin ci shaencleie = < 7 made growth. 
MERA SLCESEUOPACATSALOTIO see inte ene kn ey Aer ee oer es eek Bete 6 made growth. 
Notes. 
When tubes of different media containing the same organism from the 
same original colony were put side by side, the following was noted: 
(1) The growth on agar alone, soil and agar or on soil extract (unheated) 
was small. 
(2) The soil extract carried a better growth than the soil alone. 
(3) L. and B. agar and Na. asp. agar carried a good growth. 
(4) There was more development of distinguishing characteristics as to 
form of streaks and chromogenisis present, with the L and B agar. 
Tust Mepia Test IV. 
Samples of 6/25, 1915. 
Sample No. 7. Clean Culture Plot. 
Cover Crop Investigation. 
One ce. portions of the 1 to 400,000 dilution of this sample were plated 
on the following media: 
Lipman and Brown agar. 
Conn’s sodium asparaginate agar. 
5084—7 
