100 
General Notes. 
When tubes of different media containing the same organism from the 
same original colony are put side by side, the following is noted: 
(1) The growth on agar alone, soil and agar or on soil extract (unheated) 
is small. 
(2) The soil extract carries a better growth than the soil alone. 
(3) L. and B. agar and Na. asp. agar carry a good growth. 
(4) There is more development of distinguishing characteristics as to 
form of streaks and chromogenisis present, with the L. and B. agar. 
Tusre Mepia Test V. 
Sample of 7/16, 1915. 
Sample No. 8. Millet Plot. 
Cover Crop Investigations. 
One ce. portions of the 1 to 400,000 dilution of this sample were plated 
on the following media: 
A. 
Wheat straw extract. 
Leaf extract. 
. Starch. 
. Agar alone. 
. Ammonium nitrate. 
. Conn’s sodium asparaginate. 
. Soil. 
. Soil and starch. 
Lipman and Brown agar. 
Ammonium nitrate and starch. 
(15 gms. agar is basis of all media.) 
Colonies developing well on each media, plates II] and IV, were transferred 
to slants of other media. Tables give results of growth on these slants at 
end of 6, 10 and 14 days’ incubation at 22° Centigrade. 
