129 
As a result, the chloroplasts, chondrisomes, ete., are stained a deep blue, 
the cytoplasm a light orange or almost colorless, and the cell walls varying 
intensities of orange. 
The writer has never been able to see the use of the treatment with 
alizarin, and this part of the process may be omitted. However, Benda’s 
solution of alizarin is made as follows: Make a saturated solution of Kahl- 
baum’s alizarin-sulfo-saurem Natron (mono.) in 70 per cent. aleohol. One 
ce. of this solution is added to 80 to 100 ce. of water. 
Benda’s erystal violet solution is made as follows: 
Saturated solution crystal violet in 70% alcohol ............ 1 part. 
9; bydrochloric acid in, 70% alcohol)... 040 ne 1 part. 
JN FATUNTT TC AW EHECEN cack rn ello Ca ea Sat acetate Oe One ae 2 parts. 
5084—9 
