all but the bottom collection, which was taken in the manner described 
below and as illustrated by the figures. 
A sixteen-ounce reagent bottle (see Fig. 1) with a ground glass stopper 
was securely fastened to a block of cement weighing approximately 30 Ibs. 
The stopper was so tied that it could be partly pulled out. A strong cord 
was attached to the neck of the bottle to permit raising and lowering the 
bottle. A second cord was attached to the stopper so that when the empty 
bottle was at the bottom the stopper could be pulled as far as its fastenings 
would permit, allowing the bottle to be filled with the bottom ooze. When 
the bottle was filled the cord attached to the stopper was loosened, thus 
allowing it to snap back in place and securely close the bottle, and with 
the cord around the neck the bottle was drawn to the surface. The stopper 
and neck of the collecting bottle were rinsed off first with aleohol, then 
distilled water. The contents were then transferred to smaller reagent 
bottles, corked and sealed with paraffin to insure their being air tight. 
The contents of the collections, especially the bottom collection, were 
examined microscopically and the plants and animals that seemed alive - 
were listed. As a check, some bottles of the same collection were kept fifteen 
days in darkness and at approximately the same temperature as the lake 
bottom. Their contents were then examined and the plants and animals 
found therein were apparently as active as when first collected. The animals 
were all seen moving with more or less rapidity, the protozoans quite rapidly, 
the higher forms not so much so. Their activity increased with exposure 
to light and air. 
From the total examinations made, the following were found, demon- 
strated to be alive and classified. Nine protozoa, one rotifer, one erustacea, 
twenty algae and fourteen diatoms. 
Animals Classified afler Conn and Webster. 
Protozoa: 
Dactylasphaerium radiosum Khr. 
Difflugia globostoma Leidy. 
Amoeba proteus Ehr. 
Helizoa: 
Actinosphaerium eichornii. 
Mastigophora-flagellata: 
Peranema sp.(?) 
