14G 



tions as to the ungerminated extracts being the lightest, tlie six days 

 germination extract being the darkest, the three days germination extract 

 being a sliade between tlie two. 



100 Degrees C. — These solutions were a little slower in responding, 

 the discoloration not being apparent for 30 minutes, but showed similar 

 discolorations to those extracts I'aised to only 60 degrees C. At the end of 

 four days they were sliglitly lighter in shade than the corresponding 

 extracts at GO degrees C. 



100 Degrees C. — To avoid any chance of error, the same (luantities of 

 the extracts were again taken, but heated over the direct flame of a 

 Bunsen burner until the solutions boiled vigorously. They were then 

 cooled rapidly in the snow, after which the pyrocatechin was added. At 

 the same time two tubes of distilled water were boiled over the flame, 

 and also had the pyrocatechin added, one being cooled before the addition, 

 the other being quite warm. 



The extracts plus the pyrocatechin behaved exactly the same as the 

 extracts heated in the steam sterilizer. The solutions of distilled water 

 plus the pyrocatechin remained clear and colorless. 



100 Degrees C. — In the first set of experiments with the extracts from 

 the various parts of the flour, the white flour extract gave the weakest 

 color reaction, seeming to indicate either Aveakest or smallest quantity of 

 enzyme. An equal quantity to that used in the other experiments was 

 boiled over the direct flame for two minutes. Another quantity was taken 

 but not boiled, both had pyrocatechin added to them. The boiling caused 

 a white precipitate to form. 



The discoloration was slow in appearing, it being fully three hours 

 before there was a certainty in regard to it. Then it had a reddish 

 appearance, like apple must when exposed to the air. In twenty-four 

 hours there was a decided red color, but the boiled solution was slightly 

 darker than the unboiled. The unboiled solution also formed a precipi- 

 tate, both precipitates shoAving the coloration of the liquids. At the end 

 of three days the color remained the peculiar red, but darker, the boiled 

 one being considerably darker. 



There was next tried some white flour extract and some six days 

 germination extract, these two extracts being at the extremes of the dis- 

 colorations, the former showing the lightest, the latter the darkest in the 

 extracts, and in their action on the phenols. The extracts were placed in 

 the autoclav, and kept until thfc indicator registered a pressure of ten 



