ma a SF Med agin’ * ‘ Ns 
: ie Bye ee Y Haak Way ‘ ty i ye i =e 
SL Oe CO 
} y 
MeN sts Were these lesions due to swine plague bacteria? — (ey ae 
Nh To determine whether the microbes seen in the impure liquid culture from the | 
Yale liver were swine plague bacteria a rabbit was inoculated by means of a hypodenmie 
(: ‘ Plies 
Ans syringe beneath the skin of the thigh with about one-eighth’ of this culture. It © ~ 
if was found dead within forty-eight hours. ; . eye! 
i Locally there were blood extravasations into the connective tissue and muscles ) | ~ 
RY: of the thigh and contiguous abdominal wall. There was also a gelatinous infiltra- 
tion of the fasciz of the muscles. Lungs cedematous; spleen dark; parenchyma ° — 
berate of liver very friable. Stomach distended with food. Mucosa covered witha layer 
aa of tenacious mucus. Immense numbers of bacteria in spleen, liver, and blood from 
by the heart all showing the characteristic polar stain of the swine plague bacteria. 
in, Very few in kidneys. From the blood and spleen pure cultures in tubes of gelatine 
i ae were obtained. Seay es 
(Np These results prove not only the presence in the liver of this pig of swine plagu 
mt bacteria, but also the absence of any pathogenic properties of the large bacillus’ et 
‘ - found in all cultures from the pig. : Ge 
Rheiia Ke} An impure gelatine culture from the blood of this pig inoculated into a rabbit j 
at gave precisely the same result. The rabbit died in three days of a septiceemia due — Hi 
0 to the swine plague bacteria, which were found in abundance in the spleen, liver, 
i and blood. Cultures were equally confirmatory. Hence both blood and liver of 
$s . the pig contained these bacteria. The appended table gives the results of the inocu- 
eat) lations: 
7 4 Pig No. 402, February 19. : el: 
aes a 
: Ege an Cia ree Pa yi 
mir b. i. p.* culture, liver. oh ane gel.t cult. blood. ey 
+ ? = 
34 { i HS ? 
eri rabbit, February 24, rabbit March 2, ae 
died February 26 died March 5 : mS: 
(cults. blood, and spleen contain only swine ‘(eults. liver, blood, contai 7 
plague bacteria). . only swine plague bacteria). d 
A pig (No. 377), after being deprived of food for nearly a day, was fed February 20 
1 ae with portions of the spleen, large and small intestine, of No,402. The handlingofthe ~~ 
q animal resulted in slight lameness for a few days. Its appetite became poor. Ina — 
t month after feeding (March 23) it was in a dying condition, and was consequently 
killed for examination. 
The post-mortem examination gave no clue as to the nature of the disease. There. 
were no indications of hog cholera. There were no specific lesions referable to 
swine plague. Previous as well as subsequent experiments have convinced us that 
the specific lung lesions of swine plague can not be produced by feeding. The le- 
sions found are briefly as follows: 
Superficial inguinal glands very large, infiltrated with a pale serum; cortex of ~ 
r,! some of the lobules contain extravasated blood, medullary portion whitish, lardace- 
ait ous. Peritonitis indicated by very slender threacs of fibrine stretched acrossthe  — 
coils of intestine. Lungs and digestive tract normal. Right ventricle distended 
with a clot, the center of which is pale. Two liquid cultures from peritoneal fluid 
remained sterile. Sw 
No. 405 was found dead February 18, after several days of great weakness and 
diarrhea. The severest lesions were confined to the lungs and the large intestines; 
as the following post-mortem notes indicate : ' 
Patches of skin on the nner aspect of limbs, over the abdomen and pubic region, _ 
deeply reddened. Lymphatics of meso-colon enlarged, but pale. .The mucous | ~ 
membrane of the large intestine presents throughout a dark red, raw aspect, and is 
covered more or less entirely with a continuous layer of exudate, which readily | 
comes away. Ventricles of heart filled with very dark, partially coagulated blood. 
The ventral portion of both lungs, involving perhaps one-half their entire volume, 
hepatized and portions of it firmly adherent to the thoracic wall, the diaphragm, 
and pericardium. 
The disease seemed to involve all below a horizontal line when the lungs were 
held in the position normally occupied in the body, as if the agencies producing the 
lesion had settled into the most dependent portions and there began their patho- — 
logical action first. The portions affected were thus the small ventral lobes resting 
on the pericardium, these being lowest in the natural position of the animal, and 
eS ean, 
* Beef infusion containing 1 per cent. peptone. 
+ Beef infusion containing 1 per cent. peptone and 10 per cent. gelatine. 
