} a hs 
‘ 
ne 
in which the Saree dae is more advanced the interlobular Hens is wistanaed with ¢ Bir 
serous infiltration; the large vessels are filled with very consistent dark clots. Heart 
rather large; pericardium free; right auricle, ventricle, and large veins distended c 
with thr ombi: smaller white thrombus in left ventricle. 
Microscopic examination of the lung tissue in cover-glass preparations shows the 
presence of numerous bacteria with the polar stain in "recent lesions; in older ones 
they are rare. Other forms are present, but only in small numbers. The pleural 
exudate was made up of round cells, bound together by bundles of fibrine; it con- 
tained few bacteria. 
In transverse sections of the large intestine, where a mass of exudite is still at- 
tached, the muscular and submucous layers are intact, if we except a slight cellular © 
infiltration near the base of the crypts. ‘The mucous layer, however, is considera- 
bly changed. The surface epithelium, including a portion of the erypts of Lieber- 
kthn, is no longer distinguishable, but merges without demarcation into an exu- 
date several millimeters thick, consisting of. leucocytes imbedded in a mesh-work 
of fibrine, the whole refusing to stain. The pathological process seems to be diphthe- 
ritic in nature, the membrane being attacked from the digestive tube and not from - 
the submucous tissue. 
Pure cultures of swine-plague bacteria in tubes of gelatine were obtained from 
the pleural exudate. In each needle track a large number of colonies developed. _ 
A piece of the false membrane gave the same result. Cover-glass preparations from 
spleen and liver were negative. Two tubes of beef infusion into which bits of spleen 
had been dropped remained sterile. Two similar cultures from the liver contain 
each the bacillus butyricus, evidently of post-mortem growth. The blood from the 
heart was also free from bacteria, for two tubes of gelatine, each inoculated six or 
seven times with blood, did not develop a single colony. 
These results show that the specific microbe is not present in the internal organs, 
and can only be obtained from the diseased lungs and pleura. <A rabbit inoculated 
J t=] 
in the ear with a bit of lung tissue died within four days. There was no swelling 
or reddening of the ear. ‘Lungs deeply congested (hypostatic?). Immense num-= 
bers of swine plague bacteria in blood, spleen, and liver. Cultures from blood and 
liver containe only the same organisms. A mouse inoculated with a bit of lung 
tissue succumbed within two days. Bacteria very scarce in body. Pure cultures 
of swine plague bacteria were, however, obtained from heart’s blood. 
Pig No. 497, died February 21. 
pleural exudate | | 
| lung tissue lung tissue - blood 
spleen 
> gel. icult. ; 
; rabbit Mar. 23 mouse Mar. 23 i cults. sterile 
died Mar.27 died Mar.25 cults. sterile 
rabbit (car)Mar.5 swineplague swine plague 
gel. cult. Mar.5 no effect bacteria bacteria 
liver 
cults. contain 
Mar. 12 butyric bacilli 
Mar. 2! 
rabbit (ear) Apr. 12 
died Apr. 15 
(swine plague) 
bacteria 
No. 408 from the same farm died March 5. The post-mortem examination was 
delayed forty-eight hours, the temperature being above the freezing point a part of 
the time. } 
Skin diffusely reddened over the entire body. On buttocks tough and leathery. 
The most marked lesions found in the internal organs are briefly as follows: On sec- 
tion of kidneys six or seven petechize on each pyramid. In fundusof stomach, which 
was filled with food, some small erosions of the mucous membrane. In the large 
intestine, the mucosa of cecum dark-red, almost blackish, and covered with laree 
ragged ulcerations only 1™™ or 2™" deep. The membrane appeared as if gnawed. 
