4 
as 
it? ¥ eo see. & A Oe a ae | ’ Pa ere a 
PM yer Ny ae ero NEY Ae ee i 
AO pe SA SGU i hak SP ee RRB J ) ; { ; 
. | Sf wien A ie Pe : ‘ \r J 
Petey Sb y 
Cart 
BUREAU OF ANIMAL INDUSTRY. 499 
vay i) ‘ \ 
filled with a large thrombus, firmly adherent to papillary muscles. Left heart con- 
tains a small dark clot. Large vessels near heart and in lungs distended with white 
thrombi; inclosed part dark ciots. 
Bacterial investigations negative. One rabbit inoculated with bit of lung tissue 
in thigh remained well. Two mice inoculated with tung tissue died on the follow- 
ing day. Cultures from blood remained sterile. All but one culture from spieen 
and liver remained sterile. This contained a bacillus growing in the bottom of the 
liquid (butyricus ?). é 
In order to test the communicable character of this lung disease, two healthy 
pigs (Nos. 359, 360) were taken February 28 to the farm and penned with Nos. 
408, 409, 410. At this time two of these three were already diseased, and No. 407 had 
already died in the same yard. On March 16, No. 408, having meanwhile suc- 
cumbed to the disease, the remaining four were taken back to a clean pen on the 
experimental station. No. 409 died March 20, as already stated, and No. 359 was 
found dead March 24. It had been so weak as to be scarcely able to stand, al- 
though apparently free from cough. No skin lesions; superficial Inguinal glands 
enlarged, pale, medullary portion dotted with blood-red points. A few slender 
fibrinous strings stretched across the coils of intestine. Stomach contracted, con- 
taining about 50° of a turbid yellow liquid. The walls covered with tenacious 
bile-stained mucus. Gall bladder contracted; contains an ascaris; one in cystic 
duct. Mucosa of large intestine dark colored. In ceecum four or five large ulcers, 
one surrounding base of valve. In another, about one-half inch across the necrotic 
center, projected like a button. In colon a large number of small ragged patches 
of a thin deposit which seems to be dead epithelium. Thorax.—Lungs do not col- 
lapse when thorax is opened. The major portion of both, excepting dorsal region, 
closely adherent to chest wall. The attachment being severed without difficulty, 
both pleural surfaces are found covered with a thin grayish deposit. Small quan- 
tity of turbid liquid present in pleural cavity; diaphragm entirely but loosely adhe- 
rent to the pulmonary pleura. Portion of pericardium also adherent to pleura on 
the right side. 
When the lings were removed from thorax, all but the dorsal region found solidi- 
fied. Evenin this there were scattered hepatized lobules. The lung tissue varies in 
color from a pale toa bright red and resembles the diseased lungsalready described. 
The smaller bronchi of the affected lobes were, as a rule, occluded with more or less 
consistent whitish piugs. Bronchial glands enlarged, cortex bright red. 
Heart but slightly distended. Right ventricle tilled with a firm washed clot; 
right auricle with a dark, soft clot. Left ventricle céntains a small, dark clot ; left 
auricle the same. This latter is dotted with closely-set, bright-red petechie, as if 
sprinkled with blood. Great vessels filled with dark clots. 
Microscopic examination reveals a large number of swine plague bacteria in 
pleural exudate, immense numbers in the ling tissue. The whitish plugs in the 
smal! air tubes contain a moderate number mingled with other forms. Sections of 
the same lung tissue showed immense numbers of swine plague bacteria among 
the alveolar exudate. Bacilli were also present, but in small number. The follow- 
ing cultures were made: Of two beef infusion cultures from the pleural exudate 
one contained the swine plague bacteria only, the other in addition a streptococcus. 
With the pure culture two rabbits were inoculated by injecting one-fifthee beneath 
the skin of thethigh. Oneof them (small white) was dead next morning. <A few 
ecchymoses in the subcutis at point of inoculation. The internal organs appeared 
unchanged. Both spleen and liver contained immense numbers of the injected 
bacteria—pure cultures of which were obtained from blood of heart and liver, The 
second rabbit was#found dead on the third day. There was locally an extensive 
infiltration of the subcutis with superficial degeneration of thigh and contiguous 
abdominal muscles. Slight peritonitis. Hemorrhage into caudal lobe of right 
lung. Very few bacteria in spleen, liver, and heart’s blood. A tube culture in 
gelatine shows the colonies in a few days. 
Subsequent inoculations were made from cultures derived from the first rabbit 
Two rabbits were inoculated in the ear with a lancet dipped into the culture liquid. 
Both died on the third and fourth day after inoeulation. The swine plague bacteria 
were found in the spleen of both. : 
Of two gelatine cultures from the pleural exudate both are impure, one contain- 
ing besides the swine plague bacteria also the streptococcus, the other a chromeo- 
genous bacillus, described as bacillus luteus in the Second Annual Report of the 
Bureau (1885), Of three cultures from the spleen in beef infusion the bacillus 
luteus is present as well as the swine plague bacteria. This chromogenous organ- 
ism had penetrated into the internal organs, the streptococcus being limited to the 
pleural cavity. 
