; En BUREAU OF ANIMAL INDUSTRY. | 503 
infusion peptone culture, one day old, of swine plague bacteria, obtafned from an 
Towa outbreak. For several days after the hind limbs were stiff, appetite poor, but 
the animal fully recovered. March 28, it was transferred with some others to a pen 
infected with swine plague and with hog cholera, as subsequent deaths showed. Two 
weeks after the animal began to fail and died April 20, 
Skin along median line of abdomen deeply reddened. Subcutis over same region 
and sides, and subperitoneal tissue dotted with numerous pale-red spots of extra- 
vasation. Liver cirrhosed, contracted, pale. Digestive tract.—Mucosa along greater 
curvature of stomach dotted with small extravasations. Near cardiac orifice a 
small diverticulum was found about 1 inch in diameter. In it were from fifteen 
to twenty yellowish-white excrescences, round, removed with difficulty, and leaving 
a raw, depressed surface (diphtheritic). Caecum and upper two-thirds of colon pig- 
mented. In the latter the summits of about seven transverse ridges were covered 
with a very thin sheet of necrosed tissue. 
The lungs are the seat of recent and extensive disease. The ventral and cephalic 
‘lobe and ventral region of principal lobe of both lungs, as well as the small azygos 
lobe, are airless, of a deep red, mottled with pale-yellowish dots as in former cases; 
_ these dots correspond to the alveoli filled with exudate. ‘ 
The disease is farthest advanced in the ventral and cephalic lobes. Here the lung 
tissue is interspersed with hard, yellowish-white nodules, from the size of a pin’s 
head to that of apea, Cover-glass preparations from the various diseased lobes show 
numerous forms of bacteria, no one predominating. The remaining portions of the 
lungs on the dorsal aspect are cedematous; a frothy liquid rapidly fills up the section. 
Seattered over the entire surface of both lungs are subpleural ecchymoses. These 
are characteristic of hog cholera in its most acute form. 
The inoculations are given in the appended table and may be very briefly sum- 
marized. Plate cultures from lung tissue gave two forms, the bacteria of swine 
plague and streptococci, mentioned in former cases. A rabbit inoculated from a 
mixed culture of these two forms died in four days. The various cultures of internal 
organs of this animal contained the swine plague bacteria only, Another rabbit in- 
oculated from a pure culture of the streptococcus died in twenty days from an ab- 
scess due to the Inoculation. Two mice inoculated with lung tissue died. In one the 
bacteria of swine,plague were found in large numbers, Of three cultures of the 
spleen of the pig only one becameturbid. This contained hog-cholera bacteria cnly, 
as shown by the microscope and inoculation of mice. 
This pig had therefore the bacteria of two diseases, the lung lesions belonging to 
one, the various hemorrhagic lesions, ecchymoses, etc., belonging to the other. 
Pig No. 392. 
RT Beat REA agit) 
lung. 
a spleen. plate cult. 
2 mice April 23. b. i. cult. Aprfl 28 E | 
died April 25, 27 (hog cholera bacteria) b. i. cult. impure b. i. cult, 
(swine plague) contains swine plague streptococcus 
bacteria and streptococci 
2 mice, April 29, ; { 
died April 30, May 4 rabbit April 30, 
(the second of hog cholens} died May 19 
rabbit April 30, (abscess) 
died May 3, 
(swine plague) 
During March and April a few animals placed in the infected pen im which 'the 
virus of both diseases had been scattered no longer took swine plague, but suc- 
cumbed to hog cholera, as the following notes clearly indicate: 
Pig No. 390, placed in the infected pen March 22, with pigs which subsequently 
died from lung disease as well ashog cholera. The animal died quite suddenly April 
6. On examination the spleen was found very large, due to engorgement with 
blood. A moderate number of hog cholera bacteria present. Fundus of stomach 
considerably reddened, In the large intestine the mucosa is studded with irregular 
masses simulating ulcers; most of them seem to be adherent fecal masses. A few, 
when removed, leave a slightly depressed surface. Glands of meso-colon reddened; 
lungs normal, with the exception of a few scattered lobules, which are collapsed. No 
swine plague lesions observable. 
The hog cholera bacteria were obtained in pure cultures from the spleen. Their 
specific nature was tested by inoculation into mice, both of which died on the fifth 
day with characteristic lesions. 
