508 REPORT OF THE COMMISSIONER OF AGRICULTURE. 
(c) At the same time, and with the same culture with which Nos. 
383 and 385 were inoculated, two pigs (Nos. 352, 388) were inoculated 
directly into the lungs through the chest wall. No. 352 received 23° 
into the right lung with a hypodermic syringe, the skin having been 
previously disinfected with a one-fifth per cent. solution of mercuric 
chloride. No. 388 received 5“ in the same way. Both animals lost 
the use of their limbs, especially the posterior, within a day after the 
operation, remaining more or less paralyzed until the animals were 
killed, April 26. In the meantime they ate but little, while there 
were no symptoms directly referable to lung disease. Shortly be- 
fore they were killed they had almost gained control of their limbs. 
Autopsy of No, 352.—Right lung firmly adherent to chest wall and 
diaphragm by means of dense, fibrous tissue. Left lung adherent in 
a few places. On the lateral aspect of the left lung an oval mass as 
large as a small fist was found inseparably attached toit. This mass 
fluctuates, and when cut consists of a dense tibrous wall one-eighth 
inch thick, its inner surface of a deep red. The contents of this sac 
were of a putty-like consistency, greenish white, surrounded by a 
turbid fluid containing small flakes. The mass consisted of degen- 
erated pus corpuscles, in which were disseminated the swine-plague 
bacteria in moderate number. The lung tissue was merely com- 
pressed by the tumor-like mass and not diseased. A gelatine tube 
culture inoculated from the caseous contents of the abscess contained 
an immense number of colonies of swine plague bacteria in each 
needle track. The absence of other micro-organisms proves that the 
abscess was the result of the presence of the injected bacteria. The 
other organs werenormal, Cultures from the spleen remained sterile. 
In No. 388 the pleuritic adhesions of the right lung are the same; 
the left lung free. Along the lateral edge of the principal lobe of 
the right lung are two tumors, one as large as a horse-chestnut, the 
other, contiguous with it, about the size of amarble. The walls and 
contents as in 352. <A liquid and a gelatine tube culture from the 
contents contain the swine plague bacteria only. In the latter the 
colonies were innumerable in each needle track. The bacteria had 
therefore not only lived for forty-one days, but had multiplied enor- 
mously, causing the lesions described. 
On January 25 Nos, 391 and 392 received into the subcutaneous 
tissue of the thighs 4° of a beef infusion peptone culture about 
twenty-four hours old. The swine plague bacteria were obtained 
from an lowa outbreak reported in 1886. 
No. 391 became lame soon after, probably from handling. Its ap- 
polite poor at first, was restored after a week. [February 18 it was 
<illed, but no lesions were found, excepting circumscribed abscesses 
on the thighs at the points of inoculation. 
No. 392 has been dwelt upon in the preceding pages. It did not 
show any symptoms referable to the inoculation. When exposed in 
an infected pen it died, being found with extensive disease of the 
lungs. Hence the inoculation was in no sense protective. 
Pig No. 387, after a fast of more than twenty-four hours, was fed 
March 8, with about 200° of a beef infusion culture of swine plague 
bacteria, the culture being about three days in thermostat at 95° F. 
No disturbance whatever followed this feeding. The animal was 
subsequently exposed to hog cholera (April 19) and died of the acute 
form of this disease. 
There is nothing to be added to the description given in the Paes 
ceding report of the Bureau of the microscopic and biological char- 
