42 Psyche [June 
and army worm blood. The blood of Melanoplus atlanis was 
used for these four experiments and Coccobacillus acridiorum 
d’Herelle, pathogenic to grasshoppers, was the organism used for 
the artificial contamination of two of the four tissue culture slides. 
Two slides were considered as checks. They were prepared by 
mixing a drop (1/19 of a c.c.) of the grasshopper blood with a drop of 
sterile, neutral, nutrient bouillon. The first day all of the blood 
cells appeared perfectly normal. On the third day some showed 
signs of disintegration, whereas others remained normal. On 
the sixth day the cells destined to disintegrate were completely 
disorganized. The others remained normal and showed cell 
division with the formation of syncytial, tissue-like masses, 
After two weeks the cells still appeared normal and the syncytia 
had increased considerably in size. The observations were not 
continued after two weeks. Throughout the entire period the 
slides had remained perfectly sterile showing that all technical 
precautions, observed during their preparation, had been adequate. 
At no time, not even during the first day before the formation of 
fibrin, did I observe any independent movement on the part of the 
blood cells. They remained passive and the only visible inde- 
pendent activity observed consisted in cell division on and after 
the sixth day. 
The two experimental slides were prepared by mixing a drop of 
the grasshopper blood with a platinum loop-full of a 24 hour culture 
of the Coccobacillus acridiorum d’Herelle, a highly motile organism. 
The slides were examined as soon as prepared. The blood cells 
appeared to be perfectly normal and remained entirely passive. 
The preparations were swarming with the motile bacteria and in 
ten to twenty minutes many of the bacteria made their way into 
the cytoplasm of the blood cells. The latter did not engulf the 
bacteria which seemed to bore their way into the cytoplasm.! 
On the third day the bacteria were no longer motile. They seemed 
to be multiplying, but appeared in bunches simulating agglutina- 
tion masses. Some of the blood cells had disintegrated; others 
appeared perfectly normal and bacteria were no longer visible 
within the cytoplasm. On the sixth day the bacteria seemed 
to be in about the same condition; multiplying, bunched and 
1 This may have been due to surface tension. It may be called phagocytosis if the word is. 
used in a broad sense. 
