NEW PROCESS FOR EXAMINING THE BRAIN STRUCTURE. 189 



and appearance connected with fibres ? I can give no definite 

 answer. I have observed that the cells in any given space 

 that are unconnected with these processes are about equal in 

 number to the terminal fibres, which appear to end in- 

 definitely or appear unconnected with the cells, though there 

 may be a slight excess in number on the side of the fibres. 

 The more intensely the preparation is stained, the greater the 

 number of those connections is there to be seen. I am there- 

 fore, inclined to believe that it is owing to the defective 

 method of examination that we are still unable to assert that 

 the fibres uniformly terminate in cells. 



With regard to the statement of Dr. Obersteiner that there 

 are nuclei of two kinds in the pure grey layer of Kolliker, 

 I may state that I believe such to be the case. Firstly, there 

 are those cells which are surrounded by a protoplasmic 

 substance, and which are the most numerous ; these are the 

 cells which are connected with the fibres from the cells of 

 Purkinje ; and, secondly, there are undoubtedly others around 

 which no protoplasm is to be seen ; these are either round or 

 more frequently slightly elongated, and both Dr. Obersteiner 

 and Dr. Meynert regard them as free nuclei belonging to the 

 neuroglia. In my preparation small arteries and veins may 

 be detected, but no capillaries, or, if any, very few, and these 

 but faintly indicated ; but by taking a freshly stained portion 

 of cerebellum and teasing it, the nuclei of the capillaries may 

 be readily detected, and will be found to resemble in appear- 

 ance, shape, and size the second kind of nuclei referred to 

 above. I am therefore of opinion that this kind of nucleus 

 belongs to the capillaries, and is not an element of the 

 neuroglia. That such is the case, I think, derives support 

 from the fact that their numbers are about what such an 

 origin would readily account for. 



The annexed chromo-lithographs represent with great 

 accuracy the appearances seen in the preparations, both with 

 regard to form and to colour. Fig 1 is magnified 140 dia- 

 meters. It includes a part of every layer of the cortex of 

 the human cerebellum. 



Fig 2 is a highly magnified view (950 diam.) of a part of 

 the pure grey layer of the cerebellum taken at a spot rather 

 nearer to the pia mater than to the layer of Purkinje's cells. 

 This figure is, to a certain degree, diagrammatic. The most 

 central process, with its attached cell, which really occupied 

 a position below and to the left of the structures represented, 

 has been inserted in place of a fibre which did not show any- 

 thing noteworthy. There was in the preparation, in the left 

 upper corner, a small rent ; this has not been represented, a 



