57 
Lymphatics in Central tendon of diaphragms.—This is most 
conveniently prepared from arabbit. The tendon is cut out 
whole from the animal, which must be still quite hot, care 
being taken not to stretch or scratch it, and that as little 
blood as possible falls on it. To ensure these conditions, 
I find it best to kill by decapitation, and allow the blood 
to drain for a short time. The thorax and abdomen are both 
laid open rapidly. The tendon can then be easily cut out 
without injury. The tendon is removed to a saucer-full of 
distilled water, at about blood-heat, and is rapidly brushed 
pretty hard with a large camel’s hair pencil on both sides for 
about a quarter ofa minute. It is then removed to the silver 
solution, in which it should be treated as the membrane from 
the frog just described. The writer generally cuts a tendon 
into three pieces, and allows one piece to remain in the 
solution two minutes, another two and a half, and the third 
three, thus obtaining preparation of various tints; and 
making sure of at least one goodone. As soon as the tendon 
has become of a light brown colour, if the process has been 
successful, the larger lymph vessels should be plainly visible 
to the naked eye white onadark ground. Itadds very much to 
the beauty of the preparation to remove the fibrous layer of 
the pleura, and that of the peritoneum from their respective 
surfaces of the tendon after the staining. ‘This may be done 
with care under water with a pair of forceps. I have found 
that these preparations may be well stained with a solution of 
carmine in ammonia, to which a few drops of acetic acid have 
been added, so as partly to precipitate it; and I have such 
preparations mounted in glycerine, which are now nine 
months’ old, and not in the least impaired. 
CHLoRIDE oF GoLtp.—Auerbach’s plexus— For making 
gold preparations of Auerbach’s plexus, a rabbit should be 
taken, the intestines of which are still contracting peristalti- 
cally. A slight transverse cut, as shallow as possible, should 
be made on the outside of a loop of intestine, and one lip of 
this cut should be taken hold of with a pair of forceps, and 
torn away. If this operation be properly performed, a narrow 
strip, consisting of a longitudinal muscular coat and _peri- 
toneum will be obtained, and, after some practice, it is 
possible to obtain such strips a foot or more in length. The 
process is rendered easier by blowing up the intestine first ; 
but this step is not necessary. ‘The strip should be plunged, 
with haste, for fear of drying, into a solution of gold chloride 
in distilled water (ith per cent.), and allowed to remain 
in the solution from ten to fifteen minutes, by which time it 
will have assumed a pale straw colour. It should then be 
