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some of the granular contents and even some basic sarcode 
may become extruded, the body-mass and the coat still seem 
to remain closely applied to each other. 
The application of acetic acid does not seem to produce 
any very noteworthy effect, save rendering the outline of the 
nucleus more sharp and marked. No very evident contrac- 
tion of the body-mass from the coat took place. But this 
experiment I have not tried sufficiently often to rely very 
much upon its general results; and I imagine I did not suc- 
ceed in bringing this re-agent to bear with sufficient energy. 
The use of a re-agent I happened to have by me for 
another purpose, a weak solution of iodine and iodide of 
potassium, was attended with very pretty results. This re- 
agent, vigorously applied, caused an immediate contraction, 
or rather coagulation, of the sarcode body-mass into one or 
several balls, the whole coming clean away from the outer 
mantle or coat (Fig. 5); when allowed to act more slowly, 
the result of the gradual retraction of the body-mass as above 
can be-seen. If, indeed, the finding of empty coats (sce 
Fig. 4) in the material did not already prove the independent 
character of this investment, I mean its want of organic 
union with, that is, its being no mere condensed exterior, or 
thickened or consolidated and altered ectosarc to the body- 
mass, I think this experiment would demonstrate the point. 
If the rhizopod and its investment were like “‘ endosarc’’ and 
** ectosarc,” I should suppose that this experiment must also 
have given, in this regard, a similar result to the preceding. 
But this experiment, the effect of which in a single specimen 
I depict in Fig. 5, gave other curious results. As I have 
already described, the body-mass, in the majority of the ex- 
amples, presented a stratum of the pale, shiny, elliptic bodies, 
just under its periphery, and immediately beneath this, it 
presented the more or less dense stratum of bright chloro- 
phyll-granules ; and within all, generally at the side most 
remote from the pseudopodium-bearing region, it admitted 
of being seen (with patience) the elliptic “‘ nucleus.” Now 
the immediate effect of the present re-agent was, as it seems 
to me, highly curious and interesting. I have said the sar- 
code mass coagulated into one or several balls, leaving the 
mantle bare, but not only did it do so, but these balls, in 
contracting, carried with them and huddled together the elliptic 
shiny bodies, which in the normal state formed the outer 
stratum, or that the more distant from the centre—whilst, at 
the same time, the chlorophyll-granules were left outside the 
contracted sarcode balls, though they, in the normal state, 
formed the inner stratum, or that nearer the centre—thus, 
