337 
perfectly limpid and free from microzymes. The conditions 
under which the liquid in glass 5 was placed differed from 
those to which that in glass 4 was subjected in one particular 
only, viz., in the fact that the former, instead of being rinsed 
with distilled water and dried, had been superheated. The 
teaching, therefore, of the experiment was, that the germinal 
particles from which the microzymes sprung must have been 
contained either in matter adherent to the surface of the 
glass, or in the distilled water used to cleanse it, or in both. 
That the former was not without its influence is rendered 
probable by the circumstance that in glass 3, which differed 
from 2 only in having been superheated, bacteria appeared 
latest. ‘To determine this question was the purpose of the 
next experiments. 
XI.—December 1.—Pasteur’s solution was prepared with 
water obtained from a well at Stevington, in Bedfordshire, 
which was sent to the laboratory for microscopical examina- 
tion. The water in question was perfectly limpid ; but after 
it was allowed to stand, a few microzymes could be discovered 
in the surface layer. None could be detected in the rest of 
the liquid. It contained a scanty deposit in which one or 
two monera occurred. ‘The solution was distributed in five 
test glasses, the conditions being as follows:—(1) The solu- 
tion was boiled in a tube, cooled rapidly, and then poured 
into a test glass which had just been heated to 200°C. ; (2) 
sclution boiled in the same manner was transferred to a glass 
which had been rinsed and dried ; (3) the boiled solution was 
- received in a superheated glass, but just before pouring it in, 
the glass was rinsed with ordinary distilled water ; (4) the 
conditions were exactly the same, excepting that the distilled 
water used for rinsing had first been boiled ; (5) the solution 
was not boiled, but the glass in which it was placed had been 
previously superheated. ‘The five glasses were numbered in 
the order in which they have been referred to, and placed 
under one shade. On December 7, 5 was already milky, the 
turbidity being due to torula cells and bacteria; 2 and 3 also 
contained bacteria. On December 8, the turbidity of 5 had 
increased, and 3 was opalescent. There were no microzymes 
either in 1 or 4. On the 13th, there were tufts of penicil- 
lium on all the liquids; the tufts were more advanced in 
fructification in 1 and 4 than the rest, but these liquids were 
still entirely free from microzymes. The last examinations 
were made on the 21st of December, when 1 and 4 were still 
in the same condition. 
Here the two liquids in which no development of micro- 
zymes took place differed from each other in the circumstance 
