338 
that the glass in which one of them was contained (4) was 
rinsed with boiled distilled water just before it was charged, 
both glasses having been superheated. On the other hand, 
3, in which microzymes appeared, differed from 4 only in the 
omission of the boiling of the water used for cleansing. By 
the comparison of these two results we were enabled to con- 
clude that ordinary distilled water may contain the germinal 
particles of microzymes in such profusion that even so small 
a quantity as is introduced into a glass in rinsing is sufficient 
to render a relatively enormous volume of liquid fruitful. 
The following is one of a series of experiments which were 
made to confirm this result :— 
XIi.—December 3.—Pasteur’s solution prepared with the 
same (Stevington) water was distributed in six glasses, all of 
which were superheated. Of these three, marked c, were 
filled with solution which had not been boiled, the remainder, 
6, with boiled solution. ‘They were placed in pair’s, one of 
each series in each pair, in different rooms. On December 
8, the glasses c were all hazy, and found to contain in- 
numerable bacteria, 6 were perfectly transparent; as time 
went on the contrast became more and more striking in con- 
sequence of the increased turbidity of c. Subsequently tufts 
of penicillium appeared on the surfaces of all the glasses, 
which in this as in the previous experiments progressed more 
rapidly in the clear solutions than in the others. 
This experiment was repeated several times with corre- 
sponding results. 
In many preceding experiments it has been shown that 
although torula cells and penicillium appear invariably and 
without exception on all nutritive liquids of which the sur- 
faces are exposed to the air, without reference to their mode 
of preparation, no amount of exposure has any effect in deter- 
mining the evolution of microzymes. This conclusion although 
it is in complete accordance with what we have already learnt 
as to their relations both in the visible and invisible state to 
moisture, is of such importance that it seemed necessary to 
establish it by special experiments. 
XIII.—January 7.—The bent glass tube for the absorp-- 
tion of carbonic acid by potash, known as Liebig’s bulbs, 
was heated to 200° C. and filled with boiling test solution. 
It was then attached by a vulcanite connecter which had 
been previously boiled, to an aspirator. During the following 
week air was drawn through it for a few hours daily. On 
the 23rd there were numerous torula cells with submerged 
tufts of mycelium in the liquid, especially in those bulbs to 
which the air had access first, but no trace of microzymes. 
